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IN VITRO GERMINATION AND POLLEN TUBE GROWTH OF MAIZE (ZEA MAYS L.) POLLEN.: I. CALCIUM AND BORON EFFECTS

Journal Article · · Canadian Journal of Botany
DOI:https://doi.org/10.1139/b67-087· OSTI ID:2473110
Pollen grains from the single cross hybrid Wf9 × H55 were cultured on an artificial medium containing 15% sucrose and 0.6% bacto-agar supplemented with all possible combinations of three concentrations (0.00, 0.03, and 0.06%) of calcium nitrate and three concentrations (0.00, 0.01, and 0.02%) of boric acid. The combination containing 0.03% calcium nitrate and 0.01% boric acid produced the highest germination (47.9%) and the longest tube length (405 μ). The addition of either calcium nitrate or boric acid alone decreased the germination percentage and pollen tube length. The percentage of grains producing more than one pollen tube was reduced by the addition of calcium nitrate or boric acid singly or in combination. The significance of these results in relation to pollen physiology was discussed.
Research Organization:
Univ. of Florida, Gainesville, FL (United States)
Sponsoring Organization:
US Atomic Energy Commission (AEC)
OSTI ID:
2473110
Report Number(s):
ORO--3114-2(Rev.)
Journal Information:
Canadian Journal of Botany, Journal Name: Canadian Journal of Botany Journal Issue: 6 Vol. 45; ISSN 0008-4026
Country of Publication:
United States
Language:
English

References (4)

Longevity of Sugarcane and corn Pollen-A Method for Long-Distance Shipment of Sugarcane Pollen by Airplane journal May 1942
In Vitro Germination of Rye (Secale cereale L.) Pollen1 journal November 1965
Hearing in the Rat at High Frequencies journal August 1941
Pollen Tube Growth of Diploid Alfalfa in Vitro1 journal July 1963

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