Immunogenicity of ascites tumor cells following in vitro hyperthermia
The concept that host immunization may be achieved by heat-induced antigenic modifications of cancer cells and/or the release of immunogenic products by dead or dying tumor cells following in vitro heating was examined. Ehrlich ascites cells were used, inasmuch as it was claimed that in vitro hyperthermia increased the immunogenicity of these cells. Tumor cell populations of different viability were obtained by heating Ehrlich cells at 42.5 degrees, 45 degrees, or 60 degrees C. Viable and nonviable cells were separated by Ficoll-Hypaque density centrifugation; viable nonreplicating cells were obtained by treatment with mitomycin C. Cell populations of different viability after heating were left to die slowly over 3 days at 37 degrees C. Swiss TO mice were then given injections of the treated cells and/or medium. No survival benefit occurred in mice inoculated with any of these different components and then challenged with viable tumor cells. Injection of irradiated cells, however, did produce host immunity. Similarly, D23 rat hepatoma ascites cells produced host immunity after 15,000 rad but not after heating. The claim that in vitro hyperthermia increases the immunogenicity of tumor cells was not confirmed.
- Research Organization:
- University Department of Clinical Biochemistry, Royal Victoria Infirmary, Newcastle upon Tyne, England
- OSTI ID:
- 6187260
- Journal Information:
- NIDA Res. Monogr.; (United States), Journal Name: NIDA Res. Monogr.; (United States) Vol. 61; ISSN MIDAD
- Country of Publication:
- United States
- Language:
- English
Similar Records
Immunization against Ehrlich's ascites carcinoma with x-irradiated turmor cells
Analysis of protective and cytotoxic immune responses in vivo against metabolically inactivated and untreated cells of a mutagenized tumor line (requirements for tumor immunogenicity)
Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ANTIGEN-ANTIBODY REACTIONS
ANTIMITOTIC DRUGS
ANTINEOPLASTIC DRUGS
ASCITES TUMOR CELLS
BIOLOGICAL EFFECTS
BODY TEMPERATURE
CELL CULTURES
CELL CYCLE
CHEMICAL REACTIONS
DECOMPOSITION
DRUGS
ELECTROMAGNETIC RADIATION
GLYCOLYSIS
HYPERTHERMIA
IMMUNITY
IONIZING RADIATIONS
MAMMALS
MICE
MITOMYCIN
NORTH AMERICA
RADIATIONS
RODENTS
SURVIVAL CURVES
TEMPERATURE EFFECTS
TUMOR CELLS
USA
VERTEBRATES
X RADIATION