Identification of methyl coenzyme M as an intermediate in methanogenesis from acetate in Methanosarcina spp
Journal Article
·
· J. Bacteriol.; (United States)
OSTI ID:6187051
The transfer of the methyl group of acetate to coenzyme M (2-mercaptoethanesulfonic acid; HS-CoM) during the metabolism of acetate to methane was investigated in cultures of Methanosarcina strain TM-1. The organism metabolized CD/sub 3/COO/sup -/ to 83% CD/sub 3/H and 17% CD/sub 2/H/sub 2/ and produced no CDH/sub 3/ or CH/sub 4/. The isotopic composition of coenzyme M in cells grown on CD/sub 3/COO/sup -/ was analyzed with a novel gas chromatography-mass spectrometry technique. The cells contained CD/sub 3/-S-CoM and CD/sub 2/H-S-CoM) in a proportion similar to that of CD/sub 3/H to CD/sub 2/H/sub 2/. These results, in conjunction with a report that extracts of acetate-grown strain TM-1 contain high levels of CH/sub 3/-S-CoM methylreductase, indicate that CH/sub 3/-S-CoM is an intermediate in the metabolism of acetate to methane in this organism.
- Research Organization:
- Virginia Polytechnic Inst. and State Univ., Blacksburg
- OSTI ID:
- 6187051
- Journal Information:
- J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 160:2; ISSN JOBAA
- Country of Publication:
- United States
- Language:
- English
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· Proc. Natl. Acad. Sci. U.S.A.; (United States)
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Related Subjects
09 BIOMASS FUELS
090122* -- Hydrocarbon Fuels-- Preparation from Wastes or Biomass-- (1976-1989)
140504 -- Solar Energy Conversion-- Biomass Production & Conversion-- (-1989)
550700 -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
ACETATES
ALKANES
BACTERIA
BIOLOGICAL PATHWAYS
BIOSYNTHESIS
CARBOXYLIC ACID SALTS
CHEMICAL ANALYSIS
CHROMATOGRAPHY
COENZYMES
GAS CHROMATOGRAPHY
HYDROCARBONS
ISOTOPE RATIO
MASS SPECTROSCOPY
METABOLISM
METHANE
METHANOGENIC BACTERIA
MICROORGANISMS
ORGANIC COMPOUNDS
SEPARATION PROCESSES
SPECTROSCOPY
SYNTHESIS
090122* -- Hydrocarbon Fuels-- Preparation from Wastes or Biomass-- (1976-1989)
140504 -- Solar Energy Conversion-- Biomass Production & Conversion-- (-1989)
550700 -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
ACETATES
ALKANES
BACTERIA
BIOLOGICAL PATHWAYS
BIOSYNTHESIS
CARBOXYLIC ACID SALTS
CHEMICAL ANALYSIS
CHROMATOGRAPHY
COENZYMES
GAS CHROMATOGRAPHY
HYDROCARBONS
ISOTOPE RATIO
MASS SPECTROSCOPY
METABOLISM
METHANE
METHANOGENIC BACTERIA
MICROORGANISMS
ORGANIC COMPOUNDS
SEPARATION PROCESSES
SPECTROSCOPY
SYNTHESIS