Biological monitoring of oil shale products and effluents using short-term genetic analyses

Conference · Mon Jan 01 04:00:00 EST 1979

OSTI ID:6186129

Rao, T. K.; Epler, J. L.; Schmidt-Collerus, J. J.; Leffler, L.; Guerin, M. R.

The long-term health hazards such as mutagenesis, carcinogenesis, and teratogenesis due to the exposure to crude shale oil, particulate pollutants, and the leachates from raw or spent shale constitute a major concern in the development of shale oil technology. In order to monitor such biological effects, we have applied short-term genetic analyses with the exemplary test materials. The Salmonella/microsomal activation system (Ames assay) was generally applicable but only upon chemical fractionation. The Stedman liquid-liquid extraction procedure or the Sephadex gel filtration (LH-20) technique were effectively utilized. Mutagenicity analyses with various crude oils and product water have revealed biological activity in the basic (aromatic amine fractions) or in the neutral (polyaromatic hydrocarbon fraction) fractions. Extracts and chromatographically isolated materials from raw and spent shale were subjected to mutagenicity studies. Mutagenic activity was noted and correlates with the biological activity of compounds that are either identified or predicted to occur in these materials. Comparison to other energy technologies and overall health hazard of the test materials are discussed.

• Research Organization: Oak Ridge National Lab., TN (USA); Denver Research Inst., CO (USA)
• Sponsoring Organization: USDOE
• DOE Contract Number: W-7405-ENG-26
• OSTI ID: 6186129
• Report Number(s): CONF-790334--10
• Country of Publication: United States
• Language: English

Related Subjects

04 OIL SHALES AND TAR SANDS
040700 -- Oil Shales & Tar Sands-- Health & Safety
041000 -- Oil Shales & Tar Sands-- Environmental Aspects
550400 -- Genetics
560302* -- Chemicals Metabolism & Toxicology-- Microorganisms-- (-1987)
560306 -- Chemicals Metabolism & Toxicology-- Man-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINES
AROMATICS
BACTERIA
CARCINOGENESIS
CHEMICAL EFFLUENTS
DATA
DATA FORMS
ENERGY SOURCES
EXPERIMENTAL DATA
FOSSIL FUELS
FUELS
HAZARDS
HEALTH HAZARDS
HYDROCARBONS
INDUSTRY
INFORMATION
METAMORPHIC ROCKS
MICROORGANISMS
MINERAL OILS
MUTAGEN SCREENING
MUTAGENESIS
NUMERICAL DATA
OIL SHALE INDUSTRY
OIL SHALE PROCESSING PLANTS
OILS
ORGANIC COMPOUNDS
OTHER ORGANIC COMPOUNDS
PATHOGENESIS
POLYCYCLIC AROMATIC HYDROCARBONS
ROCKS
SALMONELLA
SCREENING
SHALE OIL
SHALE OIL FRACTIONS
SHALE TAR WATER
SHALES
SPENT SHALES
SYNTHETIC FUELS
SYNTHETIC PETROLEUM
TABLES
TERATOGENESIS
TESTING