Mutagenicity of nitrogen compounds from synthetic crude oils: collection, separation and biological testing

Conference · Tue Jan 01 04:00:00 EST 1980

OSTI ID:5704769

Rao, T K; Epler, J L; Guerin, M R; Clark, B R; Ho, C H

In order to determine the long range health effects such as carcinogenicity/mutagenicity/teratogenicity/toxicity, associated with the newly emerging energy technologies, we have utilized the Ames Salmonella assay to evaluate mutagenic properties of synthetic fuels. Coupling with class fractionation was necessary. Organic extraction and liquid/liquid partitioning was used to separate acidic and basic fraction. The neutral material was separated using Sephadex LH-20 gel filtration into saturated and aromatic fractions of various ring sizes. The alkaline fraction was subfractionated eluting with benzene and ethanol on a basic alumina column and then with isopropanol and acetone using a Sephadex LH-20 gel column. The frameshift strain TA-98 was utilized along with Aroclor-induced rat liver homogenate (S-9 mix) for the mutagenicity assay. The natural crude oils were slightly mutagenic, the polynucleararomatics constituting the activity, while the coal-derived fuels indicated mutagenicity associated with alkaline constituents as well as polyaromatics. Hydrotreated coal (H-coal, HDT) or Shale (Paraho-Shale oil, HDT) derived fuels were not mutagenic. Ninety percent of the mutagenic activity in alkaline fraction was recovered in the acetone subfraction. High resolution spectroscopy of this fraction indicates polycyclic aromatic primary amines along with azaarenes as organic constituents responsible for the mutagenic activity associated with shale- and coal-derived fuels.

• Research Organization: Oak Ridge National Lab., TN (USA)
• DOE Contract Number: W-7405-ENG-26
• OSTI ID: 5704769
• Report Number(s): CONF-800323-1
• Country of Publication: United States
• Language: English

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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AROMATICS
BACTERIA
CARCINOGENESIS
CHEMICAL REACTIONS
COAL LIQUEFACTION
COAL LIQUIDS
DATA
DATA FORMS
ENERGY SOURCES
EXPERIMENTAL DATA
FOSSIL FUELS
FRACTIONATION
FUELS
H-COAL PROCESS
HYDROCARBONS
HYDROGENATION
INFORMATION
ISOLATED VALUES
LIQUEFACTION
MICROORGANISMS
MINERAL OILS
MUTAGEN SCREENING
MUTAGENESIS
MUTATIONS
NUMERICAL DATA
OILS
ORGANIC COMPOUNDS
OTHER ORGANIC COMPOUNDS
PARAHO PROCESS
PATHOGENESIS
POLYCYCLIC AROMATIC HYDROCARBONS
SALMONELLA
SCREENING
SEPARATION PROCESSES
SHALE OIL
SHALE OIL FRACTIONS
SYNTHETIC FUELS
SYNTHETIC PETROLEUM
TERATOGENESIS
THERMOCHEMICAL PROCESSES
TOXICITY