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Biotransformation of ethanol to acetaldehyde by wild and mutant strains of methylotrophic yeast

Journal Article · · Microbiology (New York)
OSTI ID:61859
The conversion of ethanol to acetaldehyde by intact cells of wild and mutant strains of methylotrophic yeast Hansenula polymorpha was studied. It was established that mutations that lower the activity of aldehyde reductase and acetaldehyde dehydrogenase stimulate acetaldehyde accumulation. The highest accumulation of acetaldehyde was found in a mutant that possessed increased alcohol oxidase activity in growth on a medium with glucose. A decrease in formaldehyde dehydrogenase did not stimulate acetaldehyde accumulation. Bioconversion of ethanol to acetaldehyde was most effective at lowered temperatures due to marked suppression of catabolic alcohol oxidase inactivation, but not to the activity of this enzyme under indicated conditions. 27 refs., 4 figs., 3 tabs.
OSTI ID:
61859
Journal Information:
Microbiology (New York), Journal Name: Microbiology (New York) Journal Issue: 6 Vol. 63; ISSN 0026-2617; ISSN MIBLAO
Country of Publication:
United States
Language:
English

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