Production of acetaldehyde by Zymomonas mobilis

Journal Article · Tue Dec 01 04:00:00 EST 1987 · Appl. Environ. Microbiol.; (United States)

OSTI ID:5338152

Wecker, M S.A.; Zall, R R

Mutants of Zymomonas mobilis were selected for decreased alcohol dehydrogenase activity by using consecutively higher concentration of allyl alcohol. A mutant selected by using 100 mM allyl alcohol produced acetaldehyde at a level of 4.08 g/liter when the organism was grown in aerated batch cultures on a medium containing 4.0% (wt/wt) glucose. On the basis of the amount of glucose utilized, this level of acetaldehyde production represents nearly 40% of the maximum theoretical yield. Acetaldehyde produced during growth was continuously air stripped from the reactor. Acetaldehyde present in the exhaust stream was then trapped as the acetaldehyde-bisulfite addition product in an aqueous solution of sodium bisulfite and released by treatment with base. Acetaldehyde was found to inhibit growth of Z. mobilis at concentrations as low as 0.05% (wt/wt) acetaldehyde. An acetaldehyde-tolerant mutant of Z. mobilis was isolated after both mutagenesis with nitrosoguanidine and selection in the presence of vapor-phase acetaldehyde. The production of acetaldehyde has potential advantages over that of ethanol: lower energy requirements for production separation, efficient separation of product from dilute feed streams, continuous separation of product from the reactor, and a higher marketplace value.

• Research Organization: Cornell Univ., Ithaca, NY
• OSTI ID: 5338152
• Journal Information: Appl. Environ. Microbiol.; (United States), Journal Name: Appl. Environ. Microbiol.; (United States) Vol. 53:12; ISSN AEMID
• Country of Publication: United States
• Language: English

Related Subjects

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550700 -- Microbiology
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59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACETALDEHYDE
ALCOHOLS
ALDEHYDES
BACTERIA
BIODEGRADATION
BIOSYNTHESIS
CARBOHYDRATES
CHEMICAL REACTIONS
COMPARATIVE EVALUATIONS
DECOMPOSITION
ENZYME ACTIVITY
ENZYMES
ETHANOL
GLUCOSE
HEXOSES
HYDROXY COMPOUNDS
MICROORGANISMS
MONOSACCHARIDES
MUTAGENESIS
MUTANTS
NITROSO COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXIDOREDUCTASES
SACCHARIDES
SYNTHESIS
TOLERANCE
TOXICITY
ZYMOMONAS MOBILIS