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Protein kinase C regulates ionic conductance in hippocampal pyramidal neurons: electrophysiological effects of phorbol esters

Journal Article · · Proc. Natl. Acad. Sci. U.S.A.; (United States)
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The vertebrate central nervous system contains very high concentrations of protein kinase C, a calcium- and phospholipid-stimulated phosphorylating enzyme. Phorbol esters, compounds with inflammatory and tumor-promoting properties, bind to and activate this enzyme. To clarify the role of protein kinase C in neuronal function, the authors have localized phorbol ester receptors in the rat hippocampus by autoradiography and examined the electrophysiological effects of phorbol esters on hippocampal pyramidal neurons in vitro. Phorbol esters blocked a calcium-dependent potassium conductance. In addition, phorbol esters blocked the late hyperpolarization elicited by synaptic stimulation even though other synaptic potentials were not affected. The potencies of several phorbol esters in exerting these actions paralleled their affinities for protein kinase C, suggesting that protein kinase C regulates membrane ionic conductance.

Research Organization:
Johns Hopkins Univ., Baltimore, MD
OSTI ID:
6180956
Journal Information:
Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 82:8; ISSN PNASA
Country of Publication:
United States
Language:
English

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Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
551001 -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMAL CELLS
AUTORADIOGRAPHY
BIOLOGICAL EFFECTS
BODY
BRAIN
CARCINOGENS
CENTRAL NERVOUS SYSTEM
ELECTRIC CONDUCTIVITY
ELECTRICAL PROPERTIES
ENZYMES
ESTERS
IONIC CONDUCTIVITY
LABELLED COMPOUNDS
NERVE CELLS
NERVOUS SYSTEM
ORGANIC COMPOUNDS
ORGANS
PHORBOL ESTERS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
PHYSICAL PROPERTIES
PHYSIOLOGY
RECEPTORS
SOMATIC CELLS
TRANSFERASES
TRITIUM COMPOUNDS