Synergistic stimulation of superoxide release from neutrophils by 5-hydroxy-6,8,11,14-eicosatetraenoate (5-HETE) and mezerein requires extracellular calcium
Conference
·
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:6180329
Mezerein, an activator of Protein Kinase C, stimulates the release of large quantities of superoxide (O/sub 2//sup -/) by guinea pig peritoneal neutrophils at optimal concentrations. The calcium ionophore A23187, or the leukotriene 5-HETE, did not affect the rate of O/sub 2//sup -/ release at optimal levels of mezerein, but dramatically increased the sensitivity of these cells to suboptimal concentrations of mezerein. The concentrations of mezerein required to effect half-maximal stimulation in the presence and absence of ionophore A23187 were ca. 1 x 10/sup -10/ and 2 x 10/sup -9/M, respectively. This synergy exhibited a partial requirement for extracellular Ca/sup +2/ when ionophore A23187 was employed, and a near absolute requirement for that cation when 5-HETE was utilized. Neutrophils stimulated with an optimal amount of mezerein exhibited a translocation of Protein Kinase C activity from the soluble to a particulate fraction. This translocation was not observed in cells simulated synergistically. Ionophore A23187 and 5-HETE increased the binding of (/sup 3/H)phorbol 12,13-dibutyrate to neutrophils by ca. 30 and 50%, respectively.
- Research Organization:
- Harvard Medical School, Boston, MA
- OSTI ID:
- 6180329
- Report Number(s):
- CONF-870644-
- Conference Information:
- Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 46:6
- Country of Publication:
- United States
- Language:
- English
Similar Records
Selective inhibition of leukotriene C/sub 4/ synthesis in human neutrophils by ethacrynic acid
Production of metabolic products of arachidonic acid during cell-cell interactions
Activation and regulation of arachidonic acid release in rabbit peritoneal neutrophils
Journal Article
·
Thu May 29 00:00:00 EDT 1986
· Biochem. Biophys. Res. Commun.; (United States)
·
OSTI ID:5003040
Production of metabolic products of arachidonic acid during cell-cell interactions
Journal Article
·
Sat Sep 01 00:00:00 EDT 1984
· J. Allergy Clin. Immunol.; (United States)
·
OSTI ID:6269834
Activation and regulation of arachidonic acid release in rabbit peritoneal neutrophils
Thesis/Dissertation
·
Thu Dec 31 23:00:00 EST 1987
·
OSTI ID:7189609
Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALINE EARTH METAL COMPOUNDS
ANIMALS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CALCIUM COMPOUNDS
CATIONS
CHARGED PARTICLES
ENZYME ACTIVITY
ENZYME INHIBITORS
ENZYMES
GUINEA PIGS
IONS
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LEUKOCYTES
MAMMALS
MATERIALS
MEMBRANE TRANSPORT
NEUTROPHILS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
RADICALS
REACTION KINETICS
RODENTS
SECRETION
SUPEROXIDE RADICALS
SYNERGISM
TRACER TECHNIQUES
TRANSFERASES
TRITIUM COMPOUNDS
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
ALKALINE EARTH METAL COMPOUNDS
ANIMALS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CALCIUM COMPOUNDS
CATIONS
CHARGED PARTICLES
ENZYME ACTIVITY
ENZYME INHIBITORS
ENZYMES
GUINEA PIGS
IONS
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LEUKOCYTES
MAMMALS
MATERIALS
MEMBRANE TRANSPORT
NEUTROPHILS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
RADICALS
REACTION KINETICS
RODENTS
SECRETION
SUPEROXIDE RADICALS
SYNERGISM
TRACER TECHNIQUES
TRANSFERASES
TRITIUM COMPOUNDS
VERTEBRATES