Synergistic stimulation of superoxide release from neutrophils by 5-hydroxy-6,8,11,14-eicosatetraenoate (5-HETE) and mezerein requires extracellular calcium

Conference · Fri May 01 00:00:00 EDT 1987 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:6180329

Badwey, J A; Robinson, J M; Horn, W; Soberman, R J; Karnovsky, M J; Karnovsky, M L

Mezerein, an activator of Protein Kinase C, stimulates the release of large quantities of superoxide (O/sub 2//sup -/) by guinea pig peritoneal neutrophils at optimal concentrations. The calcium ionophore A23187, or the leukotriene 5-HETE, did not affect the rate of O/sub 2//sup -/ release at optimal levels of mezerein, but dramatically increased the sensitivity of these cells to suboptimal concentrations of mezerein. The concentrations of mezerein required to effect half-maximal stimulation in the presence and absence of ionophore A23187 were ca. 1 x 10/sup -10/ and 2 x 10/sup -9/M, respectively. This synergy exhibited a partial requirement for extracellular Ca/sup +2/ when ionophore A23187 was employed, and a near absolute requirement for that cation when 5-HETE was utilized. Neutrophils stimulated with an optimal amount of mezerein exhibited a translocation of Protein Kinase C activity from the soluble to a particulate fraction. This translocation was not observed in cells simulated synergistically. Ionophore A23187 and 5-HETE increased the binding of (/sup 3/H)phorbol 12,13-dibutyrate to neutrophils by ca. 30 and 50%, respectively.

• Research Organization: Harvard Medical School, Boston, MA
• OSTI ID: 6180329
• Report Number(s): CONF-870644-
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 46:6; ISSN FEPRA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALINE EARTH METAL COMPOUNDS
ANIMALS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CALCIUM COMPOUNDS
CATIONS
CHARGED PARTICLES
ENZYME ACTIVITY
ENZYME INHIBITORS
ENZYMES
GUINEA PIGS
IONS
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LEUKOCYTES
MAMMALS
MATERIALS
MEMBRANE TRANSPORT
NEUTROPHILS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
RADICALS
REACTION KINETICS
RODENTS
SECRETION
SUPEROXIDE RADICALS
SYNERGISM
TRACER TECHNIQUES
TRANSFERASES
TRITIUM COMPOUNDS
VERTEBRATES