Practical and theoretical considerations in the use of the mouse foot system to derive epithelial stem cell survival parameters
A series of experiments investigating the effects of 1, 2, 4, 10, and 20 dose fractions of x rays on mouse foot skin are reported. These include data for levels of acute gross reactions from dry to full moist desquamation. We have shown the following for the irradiated epidermal stem cell population of the mouse foot. (1) Detectable proliferation was not found for regimes employing 10 daily dose fractions. (2) Significant interexperimental variation occurred which did not result from changes in cellular radiosensitivity. (3) The cell-survival equation obtained from data limited to reactions below the point of moist desquamation was not different from that obtained from higher reaction data. (4) The absolute target cell-survival levels corresponding to our mean reaction scale were established using published skin clone data and extended down to 4 x 10/sup -8/, and the clonogenic population was estimated as 0.6 to 3.0 x 10/sup 7/. (5) The relative radiobiological usefulness of the various levels of acute reactions has been characterized. (6) Skin-reaction scores obtained by averaging reactions over 7 days encompassing the peak response gave the same results with fewer readings than the conventional 22-day averaging period.
- Research Organization:
- Univ. of British Columbia, Vancouver, Canada
- OSTI ID:
- 6179672
- Journal Information:
- Radiat. Res.; (United States), Vol. 77:3
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
EPITHELIUM
STEM CELLS
SKIN
BIOLOGICAL RADIATION EFFECTS
SURVIVAL TIME
DATA ANALYSIS
EXPERIMENTAL DATA
FEET
FRACTIONATED IRRADIATION
ISOLATED VALUES
LOCAL IRRADIATION
MICE
THEORETICAL DATA
X RADIATION
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BODY
BODY AREAS
DATA
DATA FORMS
ELECTROMAGNETIC RADIATION
INFORMATION
IONIZING RADIATIONS
IRRADIATION
LEGS
LIMBS
MAMMALS
NUMERICAL DATA
ORGANS
RADIATION EFFECTS
RADIATIONS
RODENTS
SOMATIC CELLS
TISSUES
VERTEBRATES
560152* - Radiation Effects on Animals- Animals