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Transport of citrate across renal brush border membrane: effects of dietary acid and alkali loading

Journal Article · · Am. J. Physiol.; (United States)
OSTI ID:6179525
; ;
Dietary acid or alkali loading was given to rats by providing 150 mM NH4Cl or 150 mM NaHCO3 in place of drinking water for 6 days; control animals received 150 mM NaCl. After 6 days, the citrate clearance was 0.04 +/- 0.01 ml/min (mean +/- SE) in the acid-loaded group, 0.9 +/- 0.1 ml/min in the control group, and 2.5 +/- 0.2 ml/min in the alkali-loaded group. At the end of the experiment, the rats were killed, and the Na gradient-dependent citrate uptake was measured in brush border membrane (BBM) vesicles prepared from each group. At 0.3 min, the ( UC)citrate uptake was 198 +/- 8 pmol/mg protein (mean +/- SE) in the acid-loaded group, 94 +/- 16 pmol/mg protein in the control group, and 94 +/- 13 pmol/mg protein in the alkali-loaded group. The rate of Na -independent (NaCl in medium replaced by KCl) ( UC)-citrate uptake by BBM vesicles was the same for acid-loaded, control, and alkali-loaded animals. Thus, the increased capacity of the proximal tubular BBM to transport citrate from the tubular lumen into the cell interior may be an important factor that contributes to decreased urinary citrate in the presence of metabolic acidosis induced by chronic dietary acid loading.
Research Organization:
Mayo Clinic, Rochester, MN
OSTI ID:
6179525
Journal Information:
Am. J. Physiol.; (United States), Journal Name: Am. J. Physiol.; (United States); ISSN AJPHA
Country of Publication:
United States
Language:
English

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Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALI METAL COMPOUNDS
AMMONIUM CHLORIDES
AMMONIUM COMPOUNDS
AMMONIUM HALIDES
ANIMALS
BODY
CARBON 14 COMPOUNDS
CARBON COMPOUNDS
CARBONATES
CARBOXYLIC ACID SALTS
CHLORIDES
CHLORINE COMPOUNDS
CITRATES
DIET
HALIDES
HALOGEN COMPOUNDS
KIDNEYS
LABELLED COMPOUNDS
MAMMALS
MEMBRANE TRANSPORT
ORGANS
OXYGEN COMPOUNDS
PH VALUE
PHYSIOLOGY
RATS
RODENTS
SODIUM CARBONATES
SODIUM COMPOUNDS
TUBULES
VERTEBRATES