Vascular endothelial cells in cell-mediated immunity: adoptive transfer with in vitro conditioned cells is genetically restricted at the endothelial cell barrier
Delayed-type hypersensitivity (DTH) is a cell-mediated immune response that can be adoptively transferred in rats when greater than 2 X 10(8) cells from peritoneal exudate, lymph nodes, or spleen are used. We have shown that by using an in vitro conditioning step with antigen, transfer can be subsequently carried out with as few as 2 X 10(7) spleen cells. The magnitude of DTH was reflected in ear swelling after intradermal injection of antigen (tuberculin or keyhole limpet hemocyanin (KLH)) and confirmed histologically. The transfer was antigen specific, requiring the sensitizing antigen in both the in vitro conditioning step and in the ear test challenge. Adoptive transfer with conditioned cells was genetically restricted by alleles of the RT-1 region (major histocompatibility complex (MHC) of the rat). Brown Norway strain (n haplotype) immune cells would not transfer DTH to Lewis (1 haplotype), ACI (a haplotype), or Buffalo (b haplotype) rats, whereas each strain would transfer DTH to syngeneic recipients. Moreover, this pattern of restriction held for all strains when tested in reciprocal fashion. In additional experiments, F1 to parental bone marrow chimeras were constructed so that bone-marrow-derived cells and non-bone-marrow-derived cells were of different RT-1 haplotypes. When these chimeras were used as recipients, transfer of DTH was only observed when immune donor cells and recipient non-bone-marrow-derived cells were syngeneic. These results point to the critical role of non-bone-marrow-derived cells (endothelial cells) in the DTH reaction.
- Research Organization:
- Portland Veterans Administration Medical Center, OR
- OSTI ID:
- 6178618
- Journal Information:
- J. Cell. Biochem.; (United States), Journal Name: J. Cell. Biochem.; (United States) Vol. 1; ISSN JCEBD
- Country of Publication:
- United States
- Language:
- English
Similar Records
Mechanism of stimulation of antibody-forming ability of bone marrow cells of mice immunized with staphylococci
DETERMINATION OF SPECIFIC IMMUNOLOGICAL TOLERANCE IN RADIATION CHIMERAS
Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
ANTIGENS
AUDITORY ORGANS
BODY
BONE MARROW
CHIMERAS
ENDOTHELIUM
HEMATOPOIETIC SYSTEM
IMMUNE REACTIONS
LYMPH NODES
LYMPHATIC SYSTEM
MAMMALS
MOSAICISM
ORGANS
RADIATION CHIMERAS
RATS
RODENTS
SENSE ORGANS
SOMATIC CELLS
SPLEEN CELLS
TISSUES
VERTEBRATES