Requirement for cholesterol for phorbol ester-induced adhesion
Conference
·
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:6175208
The human monocyte-like cell line which is a cholesterol auxotroph, is nonadherent. However, it became adherent after treatment with phorbol myristate acetate (PMA). They investigated the effects of cellular cholesterol depletion and repletion on the effectiveness of PMA to induce adhesion. Almost 70% of cellular cholesterol is depleted by incubation of the cells for 24 hrs in the growth medium in which delipidated fetal calf serum (dlfcs) is substituted for fetal calf serum (fcs) without affecting viability or rate of growth. Cells were incubated in medium containing PMA and fcs or dlfcs. The use of dlfcs inhibited the yield of adherent cells by 40%. If the cells were preincubated in the medium containing dlfcs for 6 hrs prior to addition of PMA, adhesion was inhibited by 90%. Addition of cholesterol to the medium containing dlfcs replenished cellular cholesterol and fully restored the ability of PMA to induce adhesion. In contrast, cholesterol depletion does not inhibit PMA-induced superoxide ion production. These observations indicate a specific function for cholesterol in adhesion that is independent of PMA-induced differentiation.
- Research Organization:
- Hahnemann Univ. School of Medicine, Philadelphia, PA
- OSTI ID:
- 6175208
- Report Number(s):
- CONF-870644-
- Conference Information:
- Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 46:6
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADHESION
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL FUNCTIONS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARCINOGENS
CELL DIFFERENTIATION
CHOLESTEROL
ESTERS
FUNCTIONS
HYDROXY COMPOUNDS
LEUKOCYTES
MAMMALS
MAN
MATERIALS
MONOCYTES
ORGANIC COMPOUNDS
PHORBOL ESTERS
PRIMATES
RADICALS
STEROIDS
STEROLS
SUPEROXIDE RADICALS
VERTEBRATES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADHESION
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL FUNCTIONS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARCINOGENS
CELL DIFFERENTIATION
CHOLESTEROL
ESTERS
FUNCTIONS
HYDROXY COMPOUNDS
LEUKOCYTES
MAMMALS
MAN
MATERIALS
MONOCYTES
ORGANIC COMPOUNDS
PHORBOL ESTERS
PRIMATES
RADICALS
STEROIDS
STEROLS
SUPEROXIDE RADICALS
VERTEBRATES