Comparative efficacy of antigen and antibody detection tests for human trichinellosis

Journal Article · Wed Feb 01 00:00:00 EST 1989 · J. Parasitol.; (United States)

DOI:https://doi.org/10.2307/3282932· OSTI ID:6173509

Ivanoska, D; Cuperlovic, K; Gamble, H R; Murrell, K D

Sera collected from patients with suspected or confirmed exposure to Trichinella spiralis were tested for circulating parasite antigens and antiparasite antibodies. Using an immunoradiometric assay, excretory--secretory antigens from muscle-stage larvae of T. spiralis were detected in the sera of 47% of 62 patients with clinical trichinellosis and 13% of 39 patients without clinical signs but suspected of exposure to infected meat. In comparison, antibodies were detected using an indirect immunofluorescent test in the circulation of 100% of the 62 patients with clinical trichinellosis and 46% of the 39 patients with suspected exposure. The presence of antibodies specific to excretory-secretory products of T. spiralis muscle larvae was confirmed in the majority of the samples tested by a monoclonal antibody-based competitive inhibition assay. These results indicate that antibody detection is a more sensitive diagnostic method for human trichinellosis, but that antigen detection might be a useful confirmatory test because it is a direct demonstration of parasite products in the circulation.

• Research Organization: Institute of Endocrinology, Immunology and Nutrition, Zemun (Yugoslavia)
• OSTI ID: 6173509
• Journal Information: J. Parasitol.; (United States), Vol. 75:1
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
MONOCLONAL ANTIBODIES
SPECIFICITY
TRICHINOSIS
DIAGNOSIS
ANTIGEN-ANTIBODY REACTIONS
COMPARATIVE EVALUATIONS
FLUORESCENCE
MAN
MUSCLES
PATIENTS
RADIOIMMUNOASSAY
TRICHINELLA
ANIMALS
ANTIBODIES
ASCHELMINTHES
DISEASES
HELMINTHS
IMMUNOASSAY
IMMUNOLOGY
INFECTIOUS DISEASES
ISOTOPE APPLICATIONS
LUMINESCENCE
MAMMALS
NEMATODES
PARASITIC DISEASES
PRIMATES
RADIOASSAY
RADIOIMMUNOLOGY
TRACER TECHNIQUES
VERTEBRATES
550601* - Medicine- Unsealed Radionuclides in Diagnostics