Characterization of the detergent solubilized receptor for gastrin-releasing peptide
- Rorer Biotechnology, Inc., King of Prussia, PA (USA)
Properties of detergent solubilized gastrin-releasing peptide receptor were investigated. Swiss 3T3 membranes were covalently labeled with ({sup 125}I)GRP and homobifunctional cross-linkers. A major labeled protein of 75 kDa was resolved using SDS-polyacrylamide gel electrophoresis. When the same preparation was solubilized with zwitterionic detergent and analyzed under nondenaturing conditions the protein bound radioactivity was resolved in two different peaks, a major one of apparent molecular weight 220,000 (peak 1) and a minor one of 80,000 (peak 2) both containing the 75 kDa protein. Specific ligand binding activity also eluted with peak 1. These results indicate that the active form of bombesin/GRP receptor is a large complex containing the 75 kDa ligand binding domain.
- OSTI ID:
- 6171826
- Journal Information:
- Peptides (Fayetteville, New York); (USA), Vol. 11:4; ISSN 0196-9781
- Country of Publication:
- United States
- Language:
- English
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CELL MEMBRANES
DETERGENTS
ELECTROPHORESIS
IODINE 125
MOLECULAR WEIGHT
REAGENTS
SOLUBILITY
TRACER TECHNIQUES
ADDITIVES
BETA DECAY RADIOISOTOPES
CELL CONSTITUENTS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
EMULSIFIERS
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
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MEMBRANE PROTEINS
MEMBRANES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PROTEINS
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550201* - Biochemistry- Tracer Techniques