Solubilization of human bombesin/GRP receptors
Conference
·
· FASEB Journal (Federation of American Societies for Experimental Biology); (United States)
OSTI ID:6062405
- George Washington Univ., Washington, DC (United States)
Bombesin/gastrin releasing peptide (BN/GRP) functions as a growth factor in certain tumors. Recently, the authors found the human glioblastoma cell line U-118 binds radiolabeled (Tyr{sup 4})bombesin (BN) with high affinity (Kd = 2 nM) to a single class of sites (Bmax = 30,000/cell). Using 1 mM disuccinimidyl suberate, {sup 125}I-GRP was cross-linked to a 75 Kdalton protein using U-118 cells. Here the human BN/GRP receptor was solubilized and characterized biochemically. The data indicate that the C-terminal octapeptide of NB/GRP is essential for high affinity binding to the human BN/GRP receptor. Because the solubilized receptor has an apparent molecular weight of 200 Kdaltons using gel filtration techniques, it may be an oligomeric protein.
- OSTI ID:
- 6062405
- Report Number(s):
- CONF-9104107--
- Conference Information:
- Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (United States) Journal Volume: 4:3
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AFFINITY
ANIMAL CELLS
ANIMALS
ASTROCYTOMAS
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
DAYS LIVING RADIOISOTOPES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
GLIOMAS
GROWTH FACTORS
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MAMMALS
MAN
MEMBRANE PROTEINS
MITOGENS
NEOPLASMS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PEPTIDES
PRIMATES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RECEPTORS
TRACER TECHNIQUES
TUMOR CELLS
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
AFFINITY
ANIMAL CELLS
ANIMALS
ASTROCYTOMAS
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
DAYS LIVING RADIOISOTOPES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
GLIOMAS
GROWTH FACTORS
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MAMMALS
MAN
MEMBRANE PROTEINS
MITOGENS
NEOPLASMS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PEPTIDES
PRIMATES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RECEPTORS
TRACER TECHNIQUES
TUMOR CELLS
VERTEBRATES