Receptors for GRP/bombesin-like peptides in the rat forebrain

Journal Article · Tue Jan 01 04:00:00 EST 1985 · Peptides (Fayetteville, N.Y.); (United States)

DOI:https://doi.org/10.1016/0196-9781(85)90018-X· OSTI ID:6083846

Wolf, S S; Moody, T W

Binding sites in the rat forebrain were characterized using ( SVI-Tyr4)bombesin as a receptor probe. Pharmacology experiments indicate that gastrin releasing peptide (GRP) and the GRP fragments GRP as well as Ac-GRP inhibited radiolabeled (Tyr4)bombesin binding with high affinity. Biochemistry experiments indicated that heat, N-ethyl maleimide or trypsin greatly reduced radiolabeled (Tyr4)bombesin binding. Also, autoradiographic studies indicated that highest grain densities were present in the stria terminalis, periventricular and suprachiasmatic nucleus of the hypothalamus, dorsomedial and rhomboid thalamus, dentate gyrus, hippocampus and medial amygdaloid nucleus. The data suggest that CNS protein receptors, which are discretely distributed in the rat forebrain, may mediate the action of endogenous GRP/bombesin-like peptides.

• Research Organization: George Washington Univ. School of Medicine and Health Sciences, Washington, DC
• OSTI ID: 6083846
• Journal Information: Peptides (Fayetteville, N.Y.); (United States), Journal Name: Peptides (Fayetteville, N.Y.); (United States); ISSN PPTDD
• Country of Publication: United States
• Language: English

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