Stereospecific inositol 1,4,5-( sup 32 P)trisphosphate binding to isolated rat liver nuclei: Evidence for inositol trisphosphate receptor-mediated calcium release from the nucleus
- Centre de Neurochimie, Centre National de la Recherche Scientifique, Strasbourg (France)
It is well known that inositol 1,4,5-trisphosphate binding and release of calcium are mediated by the same protein. Several reports have indicated the location of the inositol 1,4,5-trisphosphate receptor in organelles other than endoplasmic reticulum. Immunocytochemical studies on the subcellular localization of 1,4,5-trisphosphate receptor in the Purkinje cells from two laboratories have given contradictory results regarding the nuclear location of this receptor. In this paper, a high-affinity inositol 1,4,5-({sup 32}P)trisphosphate binding site (Kd = 0.11 nM) on nuclei isolated from rat liver and devoid of any microsomal, mitochondrial, or plasma membrane constituents is documented. Furthermore, we present data demonstrating that inositol 1,4,5-trisphosphate is capable of releasing {sup 45}Ca{sup 2+} from the intact isolated liver nuclei. A rapid and transient release of calcium that was taken up by nuclei in the presence of ATP is observed. The role of inositol 1,4,5-trisphosphate in the coupling between cytoplasmic second messengers and nuclear events activated during signal transduction is postulated.
- OSTI ID:
- 6171295
- Journal Information:
- Proceedings of the National Academy of Sciences of the United States of America; (USA), Vol. 87:23; ISSN 0027-8424
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CALCIUM COMPOUNDS
SECRETION
PHOSPHOLIPIDS
BIOCHEMICAL REACTION KINETICS
RECEPTORS
BIOLOGICAL LOCALIZATION
ATP
CALCIUM 45
CELL MEMBRANES
CELL NUCLEI
LIVER
MICROSOMES
PHOSPHORUS 32
RATS
TRACER TECHNIQUES
ALKALINE EARTH ISOTOPES
ALKALINE EARTH METAL COMPOUNDS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CALCIUM ISOTOPES
CELL CONSTITUENTS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
ESTERS
EVEN-ODD NUCLEI
GLANDS
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIGHT NUCLEI
LIPIDS
MAMMALS
MEMBRANE PROTEINS
MEMBRANES
NUCLEI
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANOIDS
ORGANS
PHOSPHORUS ISOTOPES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RIBOSOMES
RODENTS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques