Combined effects of radiation and caffeine on embryonic development in mice
The combined effect of radiation and caffeine has been studied in mouse embryos. Radiation and/or caffeine were administered to ICR mice on Day 11 of gestation. Intrauterine death, gross malformation, and fetal body weight were selected as indicators of effects. Doses of whole-body gamma irradiation were 0.5 to 2.5 Gy and those of caffeine were 100 and 250 mg/kg maternal body wt. Intrauterine mortality increased with increasing radiation dose; this trend was more remarkable in combination with caffeine. Gross malformations such as cleft palate and defects of forelegs and hindlegs appeared frequently in the fetuses treated with both radiation and caffeine. Decreased fetal weight was observed even in mice treated with 0.5 Gy of radiation or 100 mg/kg caffeine. There was a linear relationship between dose and reduction of fetal weight. The fetal weight was a sensitive, precise, and easy-to-handle indicator for the effects of growth retardation. Intrauterine mortality and frequencies of cleft palate and defects of forelegs and hindlegs were higher than the sum of those induced by radiation and by caffeine separately. The results indicated that the combined action of radiation and caffeine on intrauterine death and malformations was synergistic.
- Research Organization:
- Univ. of Tokyo (Japan)
- OSTI ID:
- 6171230
- Journal Information:
- Radiat. Res.; (United States), Vol. 117:2
- Country of Publication:
- United States
- Language:
- English
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CAFFEINE
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EMBRYOS
BIOLOGICAL RADIATION EFFECTS
ONTOGENESIS
INHIBITION
DOSE-RESPONSE RELATIONSHIPS
GAMMA RADIATION
MICE
PREGNANCY
SYNERGISM
WHOLE-BODY IRRADIATION
ANALEPTICS
ANIMALS
AROMATICS
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CENTRAL NERVOUS SYSTEM AGENTS
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EXTERNAL IRRADIATION
HETEROCYCLIC COMPOUNDS
IONIZING RADIATIONS
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ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
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RADIATIONS
RODENTS
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560152* - Radiation Effects on Animals- Animals