Combined effects of radiation and caffeine on embryonic development in mice

Journal Article · Wed Feb 01 04:00:00 EST 1989 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3577328· OSTI ID:6171230

Kusama, T; Sugiura, N; Kai, M; Yoshizawa, Y

The combined effect of radiation and caffeine has been studied in mouse embryos. Radiation and/or caffeine were administered to ICR mice on Day 11 of gestation. Intrauterine death, gross malformation, and fetal body weight were selected as indicators of effects. Doses of whole-body gamma irradiation were 0.5 to 2.5 Gy and those of caffeine were 100 and 250 mg/kg maternal body wt. Intrauterine mortality increased with increasing radiation dose; this trend was more remarkable in combination with caffeine. Gross malformations such as cleft palate and defects of forelegs and hindlegs appeared frequently in the fetuses treated with both radiation and caffeine. Decreased fetal weight was observed even in mice treated with 0.5 Gy of radiation or 100 mg/kg caffeine. There was a linear relationship between dose and reduction of fetal weight. The fetal weight was a sensitive, precise, and easy-to-handle indicator for the effects of growth retardation. Intrauterine mortality and frequencies of cleft palate and defects of forelegs and hindlegs were higher than the sum of those induced by radiation and by caffeine separately. The results indicated that the combined action of radiation and caffeine on intrauterine death and malformations was synergistic.

• Research Organization: Univ. of Tokyo (Japan)
• OSTI ID: 6171230
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 117:2; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANALEPTICS
ANIMALS
AROMATICS
AZAARENES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CAFFEINE
CENTRAL NERVOUS SYSTEM AGENTS
DOSE-RESPONSE RELATIONSHIPS
DRUGS
ELECTROMAGNETIC RADIATION
EMBRYOS
EXTERNAL IRRADIATION
GAMMA RADIATION
HETEROCYCLIC COMPOUNDS
INHIBITION
IONIZING RADIATIONS
IRRADIATION
MAMMALS
MICE
ONTOGENESIS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PREGNANCY
PURINES
RADIATION EFFECTS
RADIATIONS
RODENTS
SYNERGISM
TOXICITY
VERTEBRATES
WHOLE-BODY IRRADIATION
XANTHINES