/sup 32/P-postlabeling analysis of DNA adducts in liver of wild English sole (Parophrys vetulus) and winter flounder (Pseudopleuronectes americanus)

Journal Article · Wed Mar 01 00:00:00 EST 1989 · Cancer Res.; (United States)

OSTI ID:6170970

Varanasi, U; Reichert, W L; Stein, J E

The 1-butanol adduct enhancement version of the 32P-postlabeling assay was used to measure the levels of hepatic DNA adducts in the marine flatfish, English sole (Parophrys vetulus), sampled from the Duwamish Waterway and Eagle Harbor, Puget Sound, WA, where they are exposed to high concentrations of sediment-associated chemical contaminants and exhibit an elevated prevalence of hepatic neoplasms. Hepatic DNA was also analyzed from English sole from a reference area (Useless Bay, WA) and from reference English sole treated with organic-solvent extracts of sediments from the two contaminated sites. Autoradiograms of thin-layer chromatograms of 32P-labeled hepatic DNA digests from English sole from the contaminated sites exhibited up to three diagonal radioactive zones, which were not present in autoradiograms of thin-layer chromatogram maps of 32P-labeled DNA digests from English sole from the reference site. These diagonal radioactive zones contained several distinct spots as well as what appeared to be multiple overlapping adduct spots. The levels (nmol of adducts/mol of nucleotides) of total DNA adducts for English sole from Duwamish Waterway and Eagle Harbor were 26 +/- 28 (DS) and 17 +/- 9.6, respectively. All autoradiograms of DNA from fish from the contaminated sites exhibited a diagonal radioactive zone where DNA adducts of chrysene, benzo(a)pyrene, and dibenz(a,h)anthracene, formed in vitro using English sole hepatic microsomes, were shown to chromatograph. English sole treated with extracts of the contaminated sediments had adduct profiles generally similar to those for English sole from the respective contaminated sites.

• Research Organization: Northwest and Alaska Fisheries Center, Seattle, WA (USA)
• OSTI ID: 6170970
• Journal Information: Cancer Res.; (United States), Vol. 49:5
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
BENZANTHRACENE
TOXICITY
BENZOPYRENE
CHRYSENE
DNA ADDUCTS
RADIOASSAY
FISHES
SENSITIVITY
LIVER
NEOPLASMS
AUTORADIOGRAPHY
DNA
MICROSOMES
PHOSPHORUS 32
SEDIMENTS
THIN-LAYER CHROMATOGRAPHY
WASHINGTON
WATER POLLUTION
ADDUCTS
ANIMALS
AQUATIC ORGANISMS
AROMATICS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CELL CONSTITUENTS
CHROMATOGRAPHY
CONDENSED AROMATICS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DISEASES
FEDERAL REGION X
GLANDS
HYDROCARBONS
ISOTOPES
LIGHT NUCLEI
NORTH AMERICA
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANOIDS
ORGANS
PHOSPHORUS ISOTOPES
POLLUTION
RADIOISOTOPES
SEPARATION PROCESSES
USA
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology
550501 - Metabolism- Tracer Techniques