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Lack of specific (/sup 3/H) prazosin binding sites in dog and rabbit cerebral arteries

Journal Article · · Life Sci.; (United States)
; ;

In order to explore the characteristics of alpha adrenergic receptors on cerebrovascular smooth muscle, specific binding sites for the alpha/sub 1/ adrenergic ligand, (/sup 3/H) prazosin, were studied in blood vessel homogenates. No specific (/sup 3/H) prazosin binding was found in either rabbit or dog cerebral arteries, but specific binding was demonstrated in the rabbit saphenous and ear arteries. In the ear artery /sup 3/H-prazosin binding was saturable with a K/sub d/ of 0.51 +/- 0.20 nM and a Bmax of 89 +/- 29 fmoles/mg protein. To confirm the adequacy of our membrane preparation, homogenates of both dog and rabbit cerebral arteries showed saturable specific binding with two different ligands: one for muscarinic receptors, (/sup 3/H)(-) quinuclidinyl benzilate (QNB) and one for alpha/sub 2/ adrenergic receptors, (/sup 3/H) yohimbine. The results of these studies demonstrate a lack of alpha/sub 1/ adrenergic receptors on cerebral blood vessels, confirming functional studies showing only a weak contractile response to norepinephrine. 29 references, 3 figures, 2 tables.

Research Organization:
Univ. of Arizona, Tucson
OSTI ID:
6168707
Journal Information:
Life Sci.; (United States), Journal Name: Life Sci.; (United States) Vol. 35:21; ISSN LIFSA
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ANTIHYPERTENSIVE AGENTS
ARTERIES
AUDITORY ORGANS
AUTONOMIC NERVOUS SYSTEM AGENTS
BIOCHEMICAL REACTION KINETICS
BLOOD FLOW
BLOOD VESSELS
BODY
BRAIN
CARDIOVASCULAR AGENTS
CARDIOVASCULAR SYSTEM
CENTRAL NERVOUS SYSTEM
CENTRAL NERVOUS SYSTEM AGENTS
CEREBRUM
CHEMICAL BONDS
DOGS
DRUGS
DYNAMIC FUNCTION STUDIES
HOMOGENATES
HYPOTENSION
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LIGANDS
MAMMALS
MUSCLES
NERVOUS SYSTEM
ORGANS
PSYCHOTROPIC DRUGS
RABBITS
REACTION KINETICS
RECEPTORS
SENSE ORGANS
SYMPATHOMIMETICS
TRACER TECHNIQUES
TRANQUILIZERS
TRITIUM COMPOUNDS
VERTEBRATES