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Locus for ozone sensitivity in Escherichia coli: ozrA

Journal Article · · Am. Soc. Microbiol., Abstr. Annu. Meet.; (United States)
OSTI ID:6168209
Ozone sensitive mutants of Escherichia coli B251 were isolated following fumigation to ozone or exposure to 1-methyl-3-nitro-1-nitrosoguanidine (NG). P1 was used to transduce ozone sensitivity from one of these mutants, MQ849, to AB1911 (arg metA) and to MQ259 (metA ma1B). Results of these experiments show that the locus for ozone sensitivity (ozrA) is located between arg and metA. However, ozrA seems to have an effect similar to those of uvrA and exrA when ultraviolet light (UV), X-ray and ozone sensitivities are considered, therefore confirming previous assumptions that there exist common steps in repair mechanisms involved with UV, X-ray and ozone. Preliminary results obtained with MQ1814 and MQ1844, two ozone sensitive mutants derived respectively from B251 and BHill by ozone fumigation, seem to indicate that there is a second gene, ozrB, controlling ozone sensitivity and that it is located to the right of malB.
Research Organization:
Universite de Montreal, Quebec
OSTI ID:
6168209
Journal Information:
Am. Soc. Microbiol., Abstr. Annu. Meet.; (United States), Journal Name: Am. Soc. Microbiol., Abstr. Annu. Meet.; (United States) Vol. 76; ISSN ASMAC
Country of Publication:
United States
Language:
English