The effects of Pasteurella haemolytica lipopolysaccharide on bovine pulmonary artery endothelial cells in cell culture

Thesis/Dissertation · Sun Jan 01 04:00:00 EST 1989

OSTI ID:6157684

Paulsen, D B

This study examined the potential role of Pasteurella haemolytica Al lipopolysaccharide (LPS) in the pathogenesis of the vascular lesions of pneumonic pasteurellosis. Bovine pulmonary artery endothelia cells (BPAEC) were the test model. The direct toxic potential of P. Haemolytica LPS on BPAEC was examined by cell detachment assays, morphologic alterations, and membrane damage as reflected in the leakage of large internal molecules such as LDH and {sup 51}Cr-labeled molecules. LPS-induced effects having potential for causing indirect vascular damage were studied and included neutrophil-adherence to and arachidonic acid-release from BPAEC. Several substances were examined for their ability to inhibit the LPS-induced cytotoxicity. Pasteurella haemolytica LPS caused direct toxic effects in BPAEC. Cell-detachment, LDH-leakage, {sup 51}Cr-leakage, and {sup 3}H-arachidonic acid-release proceeded with similar time- and dose-dependency after exposure of BPAEC to LPS. Morphologic alterations were observed as early as one-half hour after LPS-exposure and became collectively more severe with time. Neutrophil adherence to BPAEC was increased by LPS through independent effects on both cells types. The adherence required protein synthesis in both cell types.

• Research Organization: Oklahoma State Univ., Stillwater, OK (USA)
• OSTI ID: 6157684
• Country of Publication: United States
• Language: English

Related Subjects

550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADHESION
ANIMAL TISSUES
ANIMALS
ARACHIDONIC ACID
ARTERIES
BACTERIA
BACTERIAL DISEASES
BETA DECAY RADIOISOTOPES
BIOLOGICAL FUNCTIONS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD VESSELS
BODY
BODY FLUIDS
CARBOHYDRATES
CARBOXYLIC ACIDS
CARDIOVASCULAR SYSTEM
CATTLE
CELL CONSTITUENTS
CELL CULTURES
CELL MEMBRANES
CHROMIUM 51
CHROMIUM ISOTOPES
DISEASES
DOMESTIC ANIMALS
DOSE-RESPONSE RELATIONSHIPS
ELECTRON CAPTURE RADIOISOTOPES
ENDOTHELIUM
EVEN-ODD NUCLEI
FUNCTIONS
HYDROGEN COMPOUNDS
INFECTIOUS DISEASES
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LEUKOCYTES
LIPIDS
LIPOPOLYSACCHARIDES
MAMMALS
MATERIALS
MEMBRANES
MICROORGANISMS
MONOCARBOXYLIC ACIDS
NEUTROPHILS
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PATHOGENESIS
POLYSACCHARIDES
RADIOISOTOPES
RUMINANTS
SACCHARIDES
TIME DEPENDENCE
TISSUES
TOXICITY
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES