Isolation of the gene and hypothalamic of cDNA for the common precursor of gonadotropin-releasing hormone and prolactin release-inhibiting factor in human and rat

Journal Article · Wed Jan 01 00:00:00 EST 1986 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.83.1.179· OSTI ID:6155850

Adelman, J P; Mason, A J; Hayflick, J S; Seeburg, P H

Cloned cDNAs encoding the precursor protein for gonadotropin-releasing hormone (Gn-RH) and prolactin release-inhibiting factor (PIF) were isolated from libraries derived from human and rat hypothalamic mRNA. Nucleotide sequence analyses predict precursor proteins of 92 amino acids for both species and show identity between the human placental and human hypothalamic precursor proteins. Whereas the Gn-RH peptide structure is completely conserved in human and rat, the PIF domain of the precursor displays 70% interspecies homology. Genomic analyses revealed the presence of a single Gn-RH-PIF gene in human and rat containing sequences corresponding to the cDNA distributed across four exons.

• Research Organization: Genentech, Inc., San Francisco, CA
• OSTI ID: 6155850
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 83:1
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
LIBERINS
BIOCHEMISTRY
MESSENGER-RNA
DNA-CLONING
DNA SEQUENCING
ELECTROPHORESIS
GENETIC ENGINEERING
GONADOTROPINS
HYPOTHALAMUS
LH-RH
MAN
PHOSPHORUS 32
RATS
RECOMBINANT DNA
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
BRAIN
CENTRAL NERVOUS SYSTEM
CHEMISTRY
CLONING
DAYS LIVING RADIOISOTOPES
DNA
HORMONES
ISOTOPES
LIGHT NUCLEI
MAMMALS
NERVOUS SYSTEM
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
PHOSPHORUS ISOTOPES
PITUITARY HORMONES
PRIMATES
RADIOISOTOPES
RNA
RODENTS
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques