Modulation of rat testes lipid composition by hormones: Effect of PRL (prolactin) and hCG (human chorionic gonadotropin)

Journal Article · Sat Oct 01 00:00:00 EDT 1988 · American Journal of Physiology; (USA)

OSTI ID:6289910

Sebokova, E; Wierzbicki, A; Clandinin, M T ^[1]

---

1. Univ. of Alberta, Edmonton (Canada)

The effect of prolactin (PRL) and human chorionic gonadotropin (hCG) administration for 7 days on the composition and function of rat testicular plasma membrane was investigated. Refractory state in Leydig cells desensitized by hCG decreased the binding capacity for {sup 125}I-labeled hCG and also luteinizing hormone (LH)-induced adenosine 3{prime},5{prime}-cyclic monophosphate (cAMP) and testosterone production. In testicular membranes of hCG-treated animals, a depletion of cholesterol and an increase in total phospholipid content was observed after gonadotropin injection, thereby decreasing the cholesterol-to-phospholipid ratio. Injection of high doses of PRL had no effect on the binding capacity or affinity of the LH-hCG receptor but decreased the response of Leydig cells to LH in terms of cAMP and testosterone synthesis. PRL also increased total and esterified cholesterol and decreased free cholesterol and membrane phospholipid content. The fatty acid composition of testicular lipids was significantly and selectively influenced by both hormonal treatments. These observations suggest that metabolism of cholesterol and long-chain polyunsaturated fatty acids in testicular tissue is affected by chorionic gonadotropin and PRL and may provide the mechanism for regulating steroidogenic functions.

• OSTI ID: 6289910
• Journal Information: American Journal of Physiology; (USA), Journal Name: American Journal of Physiology; (USA) Vol. 255:4; ISSN 0002-9513; ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMP
ANDROGENS
ANDROSTANES
BETA DECAY RADIOISOTOPES
BIOCHEMISTRY
BIOLOGICAL EFFECTS
BODY
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL MEMBRANES
CHEMISTRY
CHOLESTEROL
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ESTERS
GONADOTROPINS
GONADS
HCG
HORMONES
HYDROXY COMPOUNDS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPES
KETONES
LH
LIPIDS
LTH
MALE GENITALS
MEMBRANES
METABOLISM
NUCLEI
NUCLEOTIDES
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
PEPTIDE HORMONES
PHOSPHOLIPIDS
PITUITARY HORMONES
RADIOISOTOPES
STEROID HORMONES
STEROIDS
STEROLS
TESTES
TESTOSTERONE