Ras p21 and other Gn proteins are detected in mammalian cell lines by (gamma-/sup 35/S)GTP gamma S binding
Journal Article
·
· Biochem. Biophys. Res. Commun.; (United States)
The presence of guanine nucleotide binding proteins in mouse and human cell lines was investigated using (gamma-/sup 35/S)GTP gamma S and (gamma-32P)GTP. Cell lysate polypeptides were separated by sodium dodecyl sulphate polyacrylamide gel electrophoresis and transferred to nitrocellulose. Incubation of the nitrocellulose blots with (gamma-/sup 35/S)GTP gamma S identified 9 distinct GTP-binding polypeptides in all lysates. One of these is the ras oncogene product, p21, as demonstrated by subsequent immunochemical staining of the nitrocellulose blots. We have shown that this procedure provides a sensitive method for detection of p21 in culture cell lines.
- Research Organization:
- Univ. of East Anglia, Norwich (England)
- OSTI ID:
- 6155768
- Journal Information:
- Biochem. Biophys. Res. Commun.; (United States), Vol. 159:3
- Country of Publication:
- United States
- Language:
- English
Similar Records
Characterization of post-translationally modified ras p21 proteins and their in vitro and in vivo guanine nucleotide interactions
Reactivity of a sulfhydryl group of the ras oncogene product p21 modulated by GTP binding
Thrombolamban, the 22-kDa platelet substrate of cyclic AMP-dependent protein kinase, is immunologically homologous with the Ras family of GTP-binding proteins
Thesis/Dissertation
·
Fri Jan 01 00:00:00 EST 1988
·
OSTI ID:6155768
Reactivity of a sulfhydryl group of the ras oncogene product p21 modulated by GTP binding
Journal Article
·
Wed Nov 05 00:00:00 EST 1986
· J. Biol. Chem.; (United States)
·
OSTI ID:6155768
+2 more
Thrombolamban, the 22-kDa platelet substrate of cyclic AMP-dependent protein kinase, is immunologically homologous with the Ras family of GTP-binding proteins
Journal Article
·
Mon Jan 01 00:00:00 EST 1990
· Proceedings of the National Academy of Sciences of the United States of America; (USA)
·
OSTI ID:6155768
+3 more
Related Subjects
59 BASIC BIOLOGICAL SCIENCES
POLYPEPTIDES
BIOCHEMICAL REACTION KINETICS
ELECTROPHORESIS
ISOTOPE DILUTION
MAN
MEMBRANE PROTEINS
MICE
MOLECULAR WEIGHT
NUCLEOTIDES
PHOSPHORUS 32
SULFUR 35
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
DAYS LIVING RADIOISOTOPES
EVEN-ODD NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIGHT NUCLEI
MAMMALS
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PEPTIDES
PHOSPHORUS ISOTOPES
PRIMATES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RODENTS
SULFUR ISOTOPES
TRACER TECHNIQUES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques
POLYPEPTIDES
BIOCHEMICAL REACTION KINETICS
ELECTROPHORESIS
ISOTOPE DILUTION
MAN
MEMBRANE PROTEINS
MICE
MOLECULAR WEIGHT
NUCLEOTIDES
PHOSPHORUS 32
SULFUR 35
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
DAYS LIVING RADIOISOTOPES
EVEN-ODD NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIGHT NUCLEI
MAMMALS
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PEPTIDES
PHOSPHORUS ISOTOPES
PRIMATES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RODENTS
SULFUR ISOTOPES
TRACER TECHNIQUES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques