Ras p21 and other Gn proteins are detected in mammalian cell lines by (gamma-/sup 35/S)GTP gamma S binding

Journal Article · Fri Mar 31 04:00:00 EST 1989 · Biochem. Biophys. Res. Commun.; (United States)

DOI:https://doi.org/10.1016/0006-291X(89)92247-X· OSTI ID:6155768

Comerford, J G; Gibson, J R; Dawson, A P; Gibson, I

The presence of guanine nucleotide binding proteins in mouse and human cell lines was investigated using (gamma-/sup 35/S)GTP gamma S and (gamma-32P)GTP. Cell lysate polypeptides were separated by sodium dodecyl sulphate polyacrylamide gel electrophoresis and transferred to nitrocellulose. Incubation of the nitrocellulose blots with (gamma-/sup 35/S)GTP gamma S identified 9 distinct GTP-binding polypeptides in all lysates. One of these is the ras oncogene product, p21, as demonstrated by subsequent immunochemical staining of the nitrocellulose blots. We have shown that this procedure provides a sensitive method for detection of p21 in culture cell lines.

• Research Organization: Univ. of East Anglia, Norwich (England)
• OSTI ID: 6155768
• Journal Information: Biochem. Biophys. Res. Commun.; (United States), Journal Name: Biochem. Biophys. Res. Commun.; (United States) Vol. 159:3; ISSN BBRCA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
DAYS LIVING RADIOISOTOPES
ELECTROPHORESIS
EVEN-ODD NUCLEI
ISOTOPE APPLICATIONS
ISOTOPE DILUTION
ISOTOPES
KINETICS
LIGHT NUCLEI
MAMMALS
MAN
MEMBRANE PROTEINS
MICE
MOLECULAR WEIGHT
NUCLEI
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PEPTIDES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
POLYPEPTIDES
PRIMATES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RODENTS
SULFUR 35
SULFUR ISOTOPES
TRACER TECHNIQUES
VERTEBRATES