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Characterization of post-translationally modified ras p21 proteins and their in vitro and in vivo guanine nucleotide interactions

Thesis/Dissertation ·
OSTI ID:5601052
Ras p21 proteins were used for studying the biosynthesis and biochemistry of oncogene products. These proteins migrate as a heterogeneous series of polypeptides as resolved by both one- and two-dimensional polyacrylamide gel electrophoresis (PAGE). Polyspecific rat serum antibody to ras p21 was used to immunoprecipitate forms of p21 synthesized in vivo in transformed mammalian cell lines. Two-dimensional PAGE of p21 resolved two distinct groups of polypeptides, one acidic (pI 4.8-5.3) is denoted the A forms and one less acidic (pI 6.5-7.0) called the B forms. The membrane-localized B forms of v-ras-Ha p21 are predominantly phosphorylated in vivo. Pulse-chase experiments using ({sup 35}S)methionine show cytosolic precursor pro-p21 is the most acidic form processed within 4 hours to multiple membrane-localized polypeptides having higher isoelectric points. Modification(s) responsible for these multiple forms are unknown. Preliminary evidence suggests acylation inhibitor, cerulenin, partially blocks processing and/or membrane localization of pro-p21. The guanine nucleotide binding activities of p21 were also investigated.
Research Organization:
State Univ. of New York, Buffalo, NY (USA)
OSTI ID:
5601052
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINES
AMINO ACIDS
ANIMALS
AROMATICS
AZAARENES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOASSAY
BIOCHEMICAL REACTION KINETICS
BIOCHEMISTRY
BIOSYNTHESIS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL MEMBRANES
CHEMICAL COMPOSITION
CHEMISTRY
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTROPHORESIS
EVEN-ODD NUCLEI
GENES
GUANINE
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
IMMUNOASSAY
IN VITRO
IN VIVO
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIGHT NUCLEI
LIPOTROPIC FACTORS
MAMMALS
MEMBRANE PROTEINS
MEMBRANES
METHIONINE
NUCLEI
ONCOGENES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PEPTIDES
POLYPEPTIDES
PROTEINS
PURINES
RADIOISOTOPES
RATS
REACTION KINETICS
RODENTS
SULFUR 35
SULFUR ISOTOPES
SYNTHESIS
TRACER TECHNIQUES
TWO-DIMENSIONAL ELECTROPHORESIS
VERTEBRATES