Effects of parathyroid hormone on cytosolic calcium in renal proximal tubular primary cultures

Journal Article · Fri Aug 01 04:00:00 EDT 1986 · Am. J. Physiol.; (United States)

OSTI ID:6154544

Hruska, K A; Goligorsky, M; Scoble, J; Tsutsumi, M; Westbrook, S; Moskowitz, D

The effects of parathyroid hormone on the cytoplasmic CaS concentration of canine renal proximal tubule cells grown in primary culture were determined using the fluorescent CaS indicator quin 2. The cultured cells exhibited responses to hormones, enzyme activities, transport functions, and morphology characteristic of the proximal convoluted tubule. Parathyroid hormone stimulated an immediate rise in cytoplasmic CaS , both in suspended cells and cells studies as a monolayer on Nuclepore filters. The rise in cytoplasmic CaS induced by the hormone was sustained for 15-30 min, was dose dependent, and was not mimicked by cyclic AMP. Removing CaS from the extracellular media markedly decreased cytoplasmic CaS and abolished the effects of parathyroid hormone on cytosolic CaS . 8-(N,N-diethylamino)octyl-3,4,5-trimethyoxybenzoate blocked the effects of the hormone on cytosolic CaS , but mitochondrial uncouplers failed to inhibit the effects of the hormone to increase cytoplasmic CaS . These studies support a role of CaS i the activation of proximal renal tubule cells by parathyroid hormone.

• Research Organization: Jewish Hospital of St. Louis, MO
• OSTI ID: 6154544
• Journal Information: Am. J. Physiol.; (United States), Journal Name: Am. J. Physiol.; (United States) Vol. 251:2; ISSN AJPHA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ALDEHYDES
ALKALINE EARTH METAL COMPOUNDS
AMP
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BODY
CALCIUM COMPOUNDS
CARBOHYDRATES
CARBON 14 COMPOUNDS
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON OXIDES
CELL CONSTITUENTS
CELL CULTURES
CHALCOGENIDES
CYTOPLASM
DAYS LIVING RADIOISOTOPES
DOGS
DOSE-RESPONSE RELATIONSHIPS
ELECTRON CAPTURE RADIOISOTOPES
ENZYME ACTIVITY
ENZYMES
FLUORESCENCE
GLUCOSE
HEXOSES
HORMONES
IMMUNOASSAY
IMMUNOLOGY
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KIDNEYS
LABELLED COMPOUNDS
LUMINESCENCE
MAMMALS
MONOSACCHARIDES
NUCLEI
NUCLEOTIDES
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
OXIDES
OXYGEN COMPOUNDS
PARATHORMONE
PEPTIDE HORMONES
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
PHYSIOLOGY
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
RADIOISOTOPES
SACCHARIDES
TRACER TECHNIQUES
TRANSFERASES
TRITIUM COMPOUNDS
TUBULES
VERTEBRATES