Characterization of elements of the kallikrein-kinin system in rainbow trout (Salmo gairdneri)

Thesis/Dissertation · Fri Jan 01 04:00:00 EST 1988

OSTI ID:6152732

Lipke, D W

Elements of kallikrein-kinin system (KKS) are found in anamniotic vertebrates, however, research has failed to substantiate the presence of a KKS in these animals. This research was conducted to verify the presence of elements of the KKS in rainbow trout. Trout branchial angiotensin-converting enzyme (ACE, kininase II) was similar to mammalian ACE in many physical, chemical and kinetic parameters. Angiotensin-converting enzyme-like activity (ACELA) was also demonstrated in the most primitive vertebrates. ACELA was consistently found in vertebrate respiratory tissues and was prevalent in salt and water exchanging organs. /sup 3/H-bradykinin, perfused through the trout gill was not metabolized, as demonstrated by high voltage paper electrophoresis. However, trout gills extracted and retained approximately 40% of the /sup 3/H-bradykinin perfused into the branchial vasculature. Gill tissue homogenates metabolized both /sup 3/H-bradykinin and the vasoactive substance produced by incubating trout plasma with glandular kallikrein.

• Research Organization: Notre Dame Univ., IN (USA)
• OSTI ID: 6152732
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
AQUATIC ORGANISMS
BIOCHEMISTRY
CHEMICAL COMPOSITION
CHEMISTRY
ELECTROPHORESIS
ENZYME ACTIVITY
ENZYMES
FISHES
GILLS
HYDROLASES
ISOTOPE APPLICATIONS
KININS
LABELLED COMPOUNDS
ORGANIC COMPOUNDS
PEPTIDE HYDROLASES
PEPTIDES
POLYPEPTIDES
PROTEINS
RESPIRATORY SYSTEM
TRACER TECHNIQUES
TRITIUM COMPOUNDS
TROUT
VERTEBRATES