Effect of endurance training on glucose transport capacity and glucose transporter expression in rat skeletal muscle

Journal Article · Sat Dec 01 04:00:00 EST 1990 · American Journal of Physiology; (USA)

OSTI ID:6150412

Ploug, T; Stallknecht, B M; Pedersen, O; Kahn, B B; Ohkuwa, T; Vinten, J; Galbo, H ^[1]

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1. Panum Institute, Copenhagen (Denmark)

The effect of 10 wk endurance swim training on 3-O-methylglucose (3-MG) uptake (at 40 mM 3-MG) in skeletal muscle was studied in the perfused rat hindquarter. Training resulted in an increase of approximately 33% for maximum insulin-stimulated 3-MG transport in fast-twitch red fibers and an increase of approximately 33% for contraction-stimulated transport in slow-twitch red fibers compared with nonexercised sedentary muscle. A fully additive effect of insulin and contractions was observed both in trained and untrained muscle. Compared with transport in control rats subjected to an almost exhaustive single exercise session the day before experiment both maximum insulin- and contraction-stimulated transport rates were increased in all muscle types in trained rats. Accordingly, the increased glucose transport capacity in trained muscle was not due to a residual effect of the last training session. Half-times for reversal of contraction-induced glucose transport were similar in trained and untrained muscles. The concentrations of mRNA for GLUT-1 (the erythrocyte-brain-Hep G2 glucose transporter) and GLUT-4 (the adipocyte-muscle glucose transporter) were increased approximately twofold by training in fast-twitch red muscle fibers. In parallel to this, Western blot demonstrated a approximately 47% increase in GLUT-1 protein and a approximately 31% increase in GLUT-4 protein. This indicates that the increases in maximum velocity for 3-MG transport in trained muscle is due to an increased number of glucose transporters.

• OSTI ID: 6150412
• Journal Information: American Journal of Physiology; (USA), Journal Name: American Journal of Physiology; (USA) Vol. 259:6 Pt 1; ISSN 0002-9513; ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
ANIMAL TISSUES
ANIMALS
BIOLOGICAL EFFECTS
BODY
CARBOHYDRATES
CARBON ISOTOPES
CONTRACTION
EXERCISE
GLUCOSE
HEXOSES
HORMONES
INSULIN
ISOTOPE APPLICATIONS
ISOTOPE DILUTION
ISOTOPES
MAMMALS
MEMBRANE TRANSPORT
METABOLISM
MONOSACCHARIDES
MUSCLES
ORGANIC COMPOUNDS
PEPTIDE HORMONES
PERFUSED TISSUES
RATS
RODENTS
SACCHARIDES
TISSUES
TRACER TECHNIQUES
VERTEBRATES