Biphasic regulation of development of the high-affinity saxitoxin receptor by innervation in rat skeletal muscle

Journal Article · Mon Nov 01 04:00:00 EST 1982 · J. Gen. Physiol.; (United States)

DOI:https://doi.org/10.1085/jgp.80.5.753· OSTI ID:6149443

Sherman, S J; Catterall, W A

Specific binding of /sup 3/H-saxitoxin (STX) was used to quantitate the density of voltage-sensitive sodium channels in developing rat skeletal muscle. In adult triceps surae, a single class of sites with a KD . 2.9 nM and a density of 21 fmol/mg wet wt was detected. The density of these high-affinity sites increased from 2.0 fmol/mg wet wt to the adult value in linear fashion during days 2-25 after birth. Denervation of the triceps surae at day 11 or 17 reduced final saxitoxin receptor site density to 10.4 or 9.2 fmol/mg wet wt, respectively, without changing KD. Denervation of the triceps surae at day 5 did not alter the subsequent development of saxitoxin receptor sites during days 5-9 and accelerated the increase of saxitoxin receptor sites during days 9-13. After day 13, saxitoxin receptor development abruptly ceased and the density of saxitoxin receptor sites declined to 11 fmol/wg wet wt. These results show that the regulation of high-affinity saxitoxin receptor site density by innervation is biphasic. During the first phase, which is independent of continuing innervation, the saxitoxin receptor density increases to 47-57% of the adult level. After day 11, the second phase of development, which is dependent on continuing innervation, gives rise to the adult density of saxitoxin receptors.

• Research Organization: Department of Pharmacology, University of Washington, Seattle
• OSTI ID: 6149443
• Journal Information: J. Gen. Physiol.; (United States), Journal Name: J. Gen. Physiol.; (United States) Vol. 80:5; ISSN JGPLA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ANIMAL GROWTH
ANIMALS
ANTIGENS
BIOCHEMICAL REACTION KINETICS
BODY
CHEMICAL BONDS
GROWTH
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
MAMMALS
MATERIALS
MUSCLES
ORGANS
PHARMACOLOGY
RATS
REACTION KINETICS
RECEPTORS
RODENTS
SKELETON
TOXIC MATERIALS
TOXINS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES