Detection of radioactively labeled proteins is quenched by silver staining methods: quenching is minimal for /sup 14/C and partially reversible for /sup 3/H with a photochemical stain
Silver staining methods for protein detection in polyacrylamide gels have a quenching effect on autoradiography and fluorography. This effect was quantitated for proteins in two-dimensional gels by microdensitometry using a computer equipped with an image processor and by scintillation counting of proteins solubilized from the gels. The original histologically derived silver stain had a quenching effect that was severe and irreversible for /sup 3/H detection and moderate for /sup 14/C detection. A silver stain based on photochemical methods had minimal quenching of /sup 14/C detection and less of a quenching effect than the histological stain for /sup 3/H detection. The /sup 3/H quenching effect was partially reversible for the photochemical stain.
- Research Organization:
- National Inst. of Mental Health, Bethesda, MD
- OSTI ID:
- 6149310
- Journal Information:
- Anal. Biochem.; (United States), Journal Name: Anal. Biochem.; (United States) Vol. 116:2; ISSN ANBCA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ACRYLAMIDE
AMIDES
AUTORADIOGRAPHY
BIOMEDICAL RADIOGRAPHY
CARBON 14 COMPOUNDS
CHEMICAL REACTIONS
COLLOIDS
DETECTION
DIAGNOSTIC TECHNIQUES
DISPERSIONS
ELEMENTS
FLUOROSCOPY
GELS
HISTOLOGICAL TECHNIQUES
IMAGES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MEDICINE
METALS
NUCLEAR MEDICINE
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHOTOCHEMICAL REACTIONS
PROTEINS
RADIOLOGY
SCINTILLATION QUENCHING
SILVER
STAINS
TRACER TECHNIQUES
TRANSITION ELEMENTS
TRITIUM COMPOUNDS