Radioactive reference proteins for the study of labelled proteins by gel electrophoresis: a ready preparation of radiolabelled calibration kits

Journal Article · Wed Jan 01 04:00:00 EST 1986 · Anal. Lett.; (United States)

DOI:https://doi.org/10.1080/00032718608069122· OSTI ID:7031669

Pellon, G; Michel, G

The use of unlabelled reference proteins as molecular weight markers in the study of radioactive proteins by polyacrylamide gel electrophoresis is rather inconvenient since protein standards should be stained whereas the studied proteins are usually detected by radioautography. A rapid, in vitro method for introducing a radioactive label into reference proteins is described: amino groups are acylated by a highly radioactive, commercially available reagent: N-succinimidyl-(2,3-/sup 3/H) propionate. Both standard and studied proteins can then be visualized on a single radioautograph; this allows direct comparison of mobilities, thus improving both convenience and accuracy of M/sub r/ determinations, and avoids some major drawbacks of staining, such as the loss of sensitivity of fluorography due to dye-induced quenching. Additionally, in vitro labelling may be used for the detection of proteins in trace amounts, as an alternative method versus silver staining procedures.

• OSTI ID: 7031669
• Journal Information: Anal. Lett.; (United States), Journal Name: Anal. Lett.; (United States) Vol. 19:13-14; ISSN ANALB
• Country of Publication: United States
• Language: English

Related Subjects

38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY
400702* -- Radiochemistry & Nuclear Chemistry-- Properties of Radioactive Materials
AUTORADIOGRAPHY
CALIBRATION
CHEMICAL PREPARATION
ELECTROPHORESIS
IN VITRO
LABELLED COMPOUNDS
LABELLING
ORGANIC COMPOUNDS
PROTEINS
SYNTHESIS
TRACE AMOUNTS
TRITIUM COMPOUNDS