Distribution of radiolabeled endotoxin with particular reference to the eye: concise communication

Journal Article · Fri Dec 31 23:00:00 EST 1982 · J. Nucl. Med.; (United States)

OSTI ID:6148987

Rosenbaum, J T; Hendricks, P A; Shively, J E; McDougall, I R

A single systemic injection of endotoxin (lipopolysaccharide or LPS) reproducibly induces a cellular infiltrate in the uveal tract of the rat eye within 24 hr. Other organs are not comparably sensitive to systemic endotoxin. One hypothesis to explain this unique sensitivity is that endotoxin is preferentially bound by ocular tissue. Researchers tested this hypothesis by studying the distribution in the rat of intravenously injected endotoxin that had been radiolabeled with /sup 99m/Tc or /sup 32/P. With either radionuclide the concentration of endotoxin per gram of tissue at a variety of times after injection ranging from 5 min to 3 hr and 45 min, was markedly less in the eye than in liver, kidney, or spleen. A study with radiolabeled albumin indicated that these differences could not be ascribed solely to the organ's blood volume. They demonstrate, therefore, that the eye does not preferentially bind endotoxin, and they are compatible with the hypothesis that endotoxin's ocular effects are indirectly mediated.

• Research Organization: Stanford Medical Center, California
• OSTI ID: 6148987
• Journal Information: J. Nucl. Med.; (United States), Journal Name: J. Nucl. Med.; (United States) Vol. 24:1; ISSN JNMEA
• Country of Publication: United States
• Language: English

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550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALBUMINS
ANIMALS
ANTIGENS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
BODY AREAS
COMPARATIVE EVALUATIONS
DAYS LIVING RADIOISOTOPES
DISTRIBUTION
ENDOTOXINS
EYES
FACE
HEAD
HOURS LIVING RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
ISOMERIC NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAMMALS
MATERIALS
METABOLISM
NORTH AMERICA
NUCLEI
ODD-EVEN NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PHYSIOLOGY
PROTEINS
RADIOISOTOPES
RADIONUCLIDE KINETICS
RATS
RODENTS
SENSE ORGANS
SENSITIVITY
TECHNETIUM 99
TECHNETIUM ISOTOPES
TISSUE DISTRIBUTION
TOXIC MATERIALS
TOXINS
TRACER TECHNIQUES
USA
VERTEBRATES
YEARS LIVING RADIOISOTOPES