Localization of mammary tumors in vivo with /sup 131/I-labeled Fab fragments of antibodies against mouse mammary epithelial (MME) antigens. [Mice]

Journal Article · Thu Oct 15 04:00:00 EDT 1981 · Cancer; (United States)

OSTI ID:6142732

Wilbanks, T ^[1]; Peterson, J A; Miller, S; Kaufman, L; Ortendahl, D; Ceriani, R L

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1. Children's Hospital, Oakland, CA

The Fab fragments of antibodies against cell-type-specific surface antigens of mouse mammary epithelial cells (MME-antigens) were used to localize mammary tumors successfully. The radioiodine-labeled anti-MME (Fab) was injected into mice carrying simulated mammary metastases, and after 24 hours the amount of label per gram of excised tissue was several times greater in the tumor than in liver, brain, lung, or muscle. With a high-purity germanium camera, mammary tumors were clearly located by the /sup 131/I-labeled anti-MME (Fab), and normalization to /sup 99m/Tc-pertechnetate distribution in the animal increased the specificity. The density of /sup 131/I-label was fourfold greater over the mammary tumor than over comparable areas of the mouse. No accumulation of /sup 131/I-anti-MME (Fab) was observed in nonmammary tumors nor in mammary tumors when labeled nonspecific Fab was used.

• OSTI ID: 6142732
• Journal Information: Cancer; (United States), Journal Name: Cancer; (United States) Vol. 48:8; ISSN CANCA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
ANTIBODIES
ANTIGEN-ANTIBODY REACTIONS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL LOCALIZATION
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BODY
BODY FLUIDS
BRAIN
CENTRAL NERVOUS SYSTEM
COUNTING TECHNIQUES
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DISEASES
GLANDS
HOURS LIVING RADIOISOTOPES
IMMUNOLOGY
INTERMEDIATE MASS NUCLEI
IODINE 131
IODINE ISOTOPES
ISOMERIC NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
KIDNEYS
LIVER
LUNGS
MAMMALS
MAMMARY GLANDS
MATERIALS
MICE
MUSCLES
NEOPLASMS
NERVOUS SYSTEM
NUCLEI
ODD-EVEN NUCLEI
ORGANS
OXYGEN COMPOUNDS
PERTECHNETATES
RADIOIMMUNOLOGY
RADIOISOTOPE SCANNING
RADIOISOTOPES
RESPIRATORY SYSTEM
RODENTS
TECHNETIUM 99
TECHNETIUM COMPOUNDS
TECHNETIUM ISOTOPES
TRANSITION ELEMENT COMPOUNDS
URINE
VERTEBRATES
WASTES
YEARS LIVING RADIOISOTOPES