Localization of mammary tumors in vivo with /sup 131/I-labeled Fab fragments of antibodies against mouse mammary epithelial (MME) antigens

Journal Article · Thu Jan 01 04:00:00 EST 1981 · Cancer (Philadelphia); (United States)

DOI:https://doi.org/10.1002/1097-0142(19811015)48:8<1768::AID-CNCR2820480813>3.0.CO;2-P· OSTI ID:5606224

Wilbanks, T; Peterson, J A; Miller, S; Kaufman, L; Ortendahl, D; Ceriani, R L

The Fab fragments of antibodies against cell-type-specific surface antigens of mouse mammary epithelial cells (MME-antigens) were used to localize mammary tumors successfully. The radioiodine-labeled anti-MME (Fab) was injected into mice carrying simulated mammary metastases, and after 24 hours the amount of label per gram of excised tissue was several times greater in the tumor than in liver, brain, lung, or muscle. Kidney showed considerable accumulation of label but this appeared to be nonspecific. Kinetic studies revealed a rapid elimination of labeled Fab in the urine with only 1% of the injected dose remaining in the entire blood pool after 24 hours. Wit a high-purity germanium camera, mammary tumors were clearly located ty the /sup 131/I-labeled anti-MME (Fab), and normalization to /sup 99m/Tc-pertechnetate distribution in the animal increased the specificity. The density of /sup 131/I-label was fourfold greater over the mammary tumor than over comparable areas of the mouse. No accumulation of /sup 131/I-anti-MME (Fab) was observed in nonmammary tumors nor in mammary tumors when labeled nonspecific Fab was used. An analogous system using an antihuman mammary epithelial antiserum is being developed for localization of breast metastases in humans.

• Research Organization: Children's Hospital Medical Center, Oakland, California
• OSTI ID: 5606224
• Journal Information: Cancer (Philadelphia); (United States), Journal Name: Cancer (Philadelphia); (United States) Vol. 48:8; ISSN CANCA
• Country of Publication: United States
• Language: English

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560172* -- Radiation Effects-- Nuclide Kinetics &amp; Toxicology-- Animals-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL TISSUES
ANIMALS
ANTIBODIES
ANTIGEN-ANTIBODY REACTIONS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL LOCALIZATION
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BODY
BODY FLUIDS
BRAIN
CENTRAL NERVOUS SYSTEM
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DISEASES
DISTRIBUTION
EPITHELIUM
GLANDS
HOURS LIVING RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
IODINE 131
IODINE ISOTOPES
ISOMERIC NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
KIDNEYS
LABELLED COMPOUNDS
LIVER
LUNGS
MAMMALS
MAMMARY GLANDS
MATERIALS
MICE
MUSCLES
NEOPLASMS
NERVOUS SYSTEM
NUCLEI
ODD-EVEN NUCLEI
ORGANS
OXYGEN COMPOUNDS
PERTECHNETATES
RADIOISOTOPES
RADIONUCLIDE KINETICS
RESPIRATORY SYSTEM
RODENTS
TECHNETIUM 99
TECHNETIUM COMPOUNDS
TECHNETIUM ISOTOPES
TISSUE DISTRIBUTION
TISSUES
TRANSITION ELEMENT COMPOUNDS
URINE
VERTEBRATES
WASTES
YEARS LIVING RADIOISOTOPES