Zonal differences in ethanol-induced impairments in receptor-mediated endocytosis of asialoglycoproteins in isolated rat hepatocytes

Journal Article · Fri Feb 01 04:00:00 EST 1991 · Hepatology (Baltimore); (USA)

DOI:https://doi.org/10.1016/0270-9139(91)92438-E· OSTI ID:6139689

Casey, C A; Kragskow, S L; Sorrell, M F; Tuma, D J ^[1]

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1. Department of Veterans Affairs Medical Center, Omaha, NE (USA)

We have shown previously that ethanol-induced defects in receptor-mediated endocytosis of asialoorosomucoid occurred as early as 1 wk after ethanol feeding. This study was undertaken as an initial attempt to establish a possible role of defective receptor-mediated endocytosis in liver injury by investigating whether differences exist in the effects of ethanol on receptor-mediated endocytosis in hepatocytes isolated from different regions of the liver. Perivenule cells, present in the distal half of the liver, are thought to be more susceptible to ethanol-induced liver injury than are the periportal cells located in the proximal half of the liver acini. For these studies, we fed male Sprague-Dawley rats for 7 days with liquid diets containing either ethanol (36% of calories) or isocaloric carbohydrate. Perivenule and periportal hepatocytes were then isolated using a digitonin-collagenase perfusion method. In control animals, cells isolated from the perivenule region bound significantly more ligand than did cells from the periportal region. Amounts of ligand internalized and degraded were also greater in perivenule than in periportal cells in these animals. After ethanol feeding, cells isolated from both the perivenule and periportal regions bound significantly less ligand than their respective controls. This impairment in surface and total binding was more pronounced in perivenule than in periportal cells. Internalization and degradation of the ligand were also more adversely affected in the centrilobular region as shown by decreases of greater than 60% in perivenule cells and by only 20% to 30% in periportal cells of ethanol-fed animals compared with controls.

• OSTI ID: 6139689
• Journal Information: Hepatology (Baltimore); (USA), Journal Name: Hepatology (Baltimore); (USA) Vol. 13:2; ISSN 0270-9139; ISSN HPTLD
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALCOHOLS
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BODY
DIGESTIVE SYSTEM
DISTRIBUTION
ETHANOL
GLANDS
GLYCOPROTEINS
HYDROXY COMPOUNDS
LIVER
LIVER CELLS
MAMMALS
MEMBRANE PROTEINS
METABOLISM
ORGANIC COMPOUNDS
ORGANS
PATHOLOGICAL CHANGES
PHAGOCYTOSIS
PROTEINS
RATS
RECEPTORS
RODENTS
SOMATIC CELLS
TISSUE DISTRIBUTION
VERTEBRATES