Ethanol-induced impairments in receptor-mediated endocytosis of asialoorosomucoid in isolated rat hepatocytes: Time course of impairments and recovery after ethanol withdrawal

Journal Article · Sat Apr 01 04:00:00 EST 1989 · Alcohol.: Clin. Exp. Res.; (United States)

DOI:https://doi.org/10.1111/j.1530-0277.1989.tb00323.x· OSTI ID:5714326

Casey, C A; Kragskow, S L; Sorrell, M F; Tuma, D J

Chronic ethanol administration markedly impairs the process of receptor-mediated endocytosis (RME) of a representative asialoglycoprotein, asialoorosomucoid (ASOR), by the liver. In this study, we further characterized these impairments by identifying the time of onset for ethanol-induced changes in RME as well as establishing the time course for recovery to normal endocytotic values after ethanol withdrawal. Ethanol administration for 3 days did not alter any aspect of endocytosis examined in this study. After feeding ethanol to rats for 7 days, however, significant decreases in amounts of ligand bound, internalized, and degraded were apparent. These impairments persisted throughout the 5-week feeding study although the effects were somewhat attenuated with more prolonged ethanol feeding. In addition, an accumulation of intracellular receptors was observed in ethanol-fed animals relative to controls after 7 days of ethanol feeding. In all cases, recovery of endocytotic values to control levels was partially completed after 2 to 3 days of refeeding control diet and was fully completed after 7 days of refeeding. These results indicate that ethanol feeding for as little as 7 days profoundly impairs the process of RME by the liver. These impairments can be reversed after refeeding control diet for 7 days.

• Research Organization: VA Medical Center, Omaha, NE (USA)
• OSTI ID: 5714326
• Journal Information: Alcohol.: Clin. Exp. Res.; (United States), Journal Name: Alcohol.: Clin. Exp. Res.; (United States) Vol. 13:2; ISSN ACRSD
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALCOHOLS
ANIMAL CELLS
ANIMALS
BIOLOGICAL RECOVERY
CHRONIC EXPOSURE
DIET
ETHANOL
HYDROXY COMPOUNDS
IN VITRO
LIVER CELLS
MAMMALS
MEMBRANE PROTEINS
ORGANIC COMPOUNDS
PHAGOCYTOSIS
PROTEINS
RATS
RECEPTORS
RECOVERY
RODENTS
SOMATIC CELLS
TOXICITY
VERTEBRATES