Enzymatic degradation of uracil-containing deoxyribonucleic acid. V. Survival of Escherichia coli and coliphages treated with sodium bisulfite
A number of mutants of Escherichia coli defective in the ung gene (structural gene for uracil-deoxyribonucleic acid (ura-DNA) glycosylase) are shown to be abnormally sensitive to treatment with sodium bisulfite when compared with congenic ung/sup +/ strains. These results provide further evidence that sodium bisulfite causes the deamination of cytosine to uracil in DNA and that ura-DNA glycosylase is required for the repair of U-G mispairs. The effect of the chemical is apparently selective with respect to base damage; coliphages containing cytosine in their DNA are inactivated by treatment with sodium bisulfite, whereas those containing hydroxymethylcytosine are not. ura-DNA glycosylase and the major apurinic-apyrimidinic endonuclease of E. coli may function in the same repair pathway, since the extent of inactivation of a congenic set of strains which are ung xth (structural gene for the major apurinic-apyrimidinic endonuclease of E. coli) or ung xth/sup +/ is the same.
- Research Organization:
- Stanford Univ., CA
- OSTI ID:
- 6138519
- Journal Information:
- J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 137:3; ISSN JOBAA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
AMINES
AZINES
BACTERIA
BACTERIOPHAGES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
CHEMICAL REACTIONS
CYTOSINE
DATA
DATA FORMS
DEAMINATION
DNA
ENZYMES
ESCHERICHIA COLI
EXPERIMENTAL DATA
GRAPHS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
INFORMATION
ISOLATED VALUES
KINETICS
MICROORGANISMS
MUTANTS
NUCLEASES
NUCLEIC ACIDS
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
OXYGEN COMPOUNDS
PARASITES
PHOSPHOTRANSFERASES
PYRIMIDINES
REACTION KINETICS
SULFITES
SULFUR COMPOUNDS
TRANSFERASES
URACILS
VIRUSES