Cellular and molecular repair of X-ray-induced damage: dependence on oxygen tension and nutritional status
Cellular and molecular repair was studied at 23/sup 0/C using split-dose recovery and alkaline elution techniques, respectively, as a function of cellular oxygen and nutrient conditions. Hypoxic cells in full medium showed a partial reduction in the level of sublethal damage (SLD) repair relative to aerated cells; the respective repair kinetics were similar with a common repair half-time of 30 min. Similarly, hypoxic cells showed a slight reduction in strand break rejoining capacity compared to aerated cells. Under nutrient deprivation, anoxic cells displayed no SLD repair or strand break repair, while aerated cells exhibited the same level of SLD and strand break repair as for well-fed cells. In addition, nutrient deprived cells at low O/sub 2/ levels displayed normal SLD and strand break repair capability. These results indicate that both nutrient and O/sub 2/ deprivation are necessary for complete inhibition of cellular and molecular repair, and low levels of O/sub 2/ can effectively reverse this inhibition.
- Research Organization:
- George Washington Univ. Medical Center, Washington, DC
- OSTI ID:
- 6135928
- Journal Information:
- Radiat. Res.; (United States), Vol. 101:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ANOXIA
RADIOSENSITIVITY EFFECTS
BIOLOGICAL REPAIR
KINETICS
NUTRITIONAL DEFICIENCY
STRAND BREAKS
RADIOINDUCTION
CARBON 14 COMPOUNDS
DNA
EXPERIMENTAL DATA
FIBROBLASTS
HAMSTERS
OXYGEN
OXYGEN ENHANCEMENT RATIO
TRITIUM COMPOUNDS
X RADIATION
ANIMAL CELLS
ANIMALS
BIOLOGICAL RECOVERY
CONNECTIVE TISSUE CELLS
DATA
ELECTROMAGNETIC RADIATION
ELEMENTS
INFORMATION
IONIZING RADIATIONS
LABELLED COMPOUNDS
MAMMALS
NONMETALS
NUCLEIC ACIDS
NUMERICAL DATA
ORGANIC COMPOUNDS
RADIATIONS
RECOVERY
REPAIR
RODENTS
SOMATIC CELLS
VERTEBRATES
560121* - Radiation Effects on Cells- External Source- (-1987)
560111 - Radiation Effects on Biochemicals- In Vitro- (-1987)