Relationship of DNA repair and chromosome aberrations to potentially lethal damage repair in X-irradiated mammalian cells

Journal Article · Tue Jan 01 04:00:00 EST 1980 · Basic Life Sci.; (United States)

DOI:https://doi.org/10.1007/978-1-4684-3842-0_18· OSTI ID:5871147

Fornace, Jr, A J; Nagasawa, H; Little, J B

By the alkaline elution technique, the repair of x-ray-induced DNA single strand breaks and DNA-protein cross-links was investigated in stationary phase, contact-inhibited mouse cells. During the first hour of repair, approximately 90% of x-ray induced single strand breaks were rejoined whereas most of the remaining breaks were rejoined more slowly during the next 5 h. The number of residual non-rejoined single strand breaks was approximately proportional to the x-ray dose at early repair times. DNA-protein cross-links were removed at a slower rate - T 1/2 approximately 10 to 12 h. Cells were subcultured at low density at various times after irradiation and scored for colony survival, and chromosome aberrations in the first mitosis after sub-culture. Both cell lethality and the frequency of chromosome aberrations decreased during the first several hours of repair, reaching a minimum level by 6 h; this decrease correlated temporally with the repair of the slowly rejoining DNA strand breaks. The possible relationship of DNA repair to changes in survival and chromosome aberrations is discussed.

• OSTI ID: 5871147
• Journal Information: Basic Life Sci.; (United States), Journal Name: Basic Life Sci.; (United States) Vol. 15; ISSN BLFSB
• Country of Publication: United States
• Language: English

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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BIOASSAY
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CELL CULTURES
CELL DIVISION
CHROMOSOMAL ABERRATIONS
DNA
DOSE-RESPONSE RELATIONSHIPS
ELECTROMAGNETIC RADIATION
IONIZING RADIATIONS
MAMMALS
MITOSIS
MUTATIONS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PROTEINS
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
SURVIVAL CURVES
TIME DEPENDENCE
VERTEBRATES
X RADIATION