Chromosomal damage observed in first postirradiation metaphases of repair-proficient and -deficient cell lines
Investigation of radiation induced damage in mutant strains of mammalian cells which show a defect in the rejoining of DNA double strand breaks provides an unique opportunity to examine the role of double strand breaks and the mechanisms of double strand break rejoining in the production of chromosome aberrations. This is particularly important, because there is increasing evidence that the DNA double strand break is the major lesion responsible for the formation of chromosome aberrations. To address this issue, the authors studied the induction of chromosome aberrations in xrs-5 cells, an x-ray sensitive strain of a Chinese hamster ovary cell line, which shows a defect in the rejoining of double strand breaks and their wild-type parent CHO-cells. Because radiosensitivity depends strongly on cellular age, the experiments were performed with synchronous cells.
- Research Organization:
- National Aeronautics and Space Administration, Moffett Field, CA (United States). Ames Research Center
- OSTI ID:
- 121782
- Report Number(s):
- N-95-32719; NASA-TM-110655; NAS-1.15:110655; CONF-9410388-; TRN: 9532726
- Resource Relation:
- Conference: 12. joint NASA/DARA-DLR life sciences program working group meeting, Moffett Field, CA (United States), 26-27 Oct 1994; Other Information: PBD: 1994; Related Information: Is Part Of Minutes of the 12th Joint NASA/DARA-DLR Life Sciences Program Working Group Meeting; White, R.J.; PB: 234 p.
- Country of Publication:
- United States
- Language:
- English
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