Solid-phase immunoassay for human immunoglobulin E: use of /sup 125/I-labeled protein A as a tracer. [/sup 125/I tracer technique]
A solid-phase immunoassay has been developed for human immunoglobulin (Ig) E. The specific binding of /sup 125/I-labeled protein A (/sup 125/I-PA) to the Fc region of rabbit IgG anti-IgE served as a quantitative measure of specific anti-IgE antibody bound to the IgE beads under optimal assay conditions. Inhibition of antibody binding by known amounts of standard IgE was reflected in a decreased binding of /sup 125/I-PA. The degree of inhibition of /sup 125/I-PA binding was related to the amount of fluid-phase IgE present and gave a standard curve which was used to determine the concentration of IgE in test samples. The sensitivity of this method and a double antibody radioimmunoassay (RIA), which was developed using the same IgE preparation and anti-IgE antibody, was approximately the same. These assays gave similar results when used to determine levels of IgE in normal human sera that had been absorbed with protein A-Sepharose to remove components responsible for specific and nonspecific interference in the assays.
- Research Organization:
- National Cancer Inst., Bethesda, MD
- OSTI ID:
- 6133374
- Journal Information:
- Anal. Biochem.; (United States), Journal Name: Anal. Biochem.; (United States) Vol. 93:1; ISSN ANBCA
- Country of Publication:
- United States
- Language:
- English
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62 RADIOLOGY AND NUCLEAR MEDICINE
ANTIBODIES
BETA DECAY RADIOISOTOPES
DATA
DATA FORMS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
EXPERIMENTAL DATA
GLOBULINS
GRAPHS
IMMUNOGLOBULINS
INFORMATION
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
NUCLEI
NUMERICAL DATA
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
SENSITIVITY
TRACER TECHNIQUES