Distribution of ascorbate-2-sulfate and distribution, half-life and turnover rates of (1-/sup 14/C)ascorbic acid in rainbow trout

Journal Article · Fri Jun 01 04:00:00 EDT 1984 · J. Nutr.; (United States)

OSTI ID:6131998

Tucker, B W; Halver, J E

Rainbow trout (250 g) were maintained at 15 degrees C for 3 months on a low ascorbic acid diet, given (1-/sup 14/C)ascorbic acid by gavage, then fed the NAS/NRC requirement 12 times per week. Total urine, fecal water and branchial water were collected daily from five fish placed in metabolism chambers for four successive 5-day periods. Tissue samples were analyzed for /sup 14/C, ascorbic acid (C1) and ascorbate-2-sulfate (C2). Excretion analysis indicated t1/2 . 42 days. After 20 days, the feeding schedule was changed to 3 times per week. Fish fed /sup 14/C were sampled after 1, 2, 3 and 4 months. The half-life in each organ except brain was inversely proportional to the dietary level of ascorbate. Concentrations of C1 and C2 in the various tissues reflected dietary intake of vitamin C. Total C (CT . C1 + C2) levels were maintained in the liver even with the low vitamin C diet. Estimates of body pool for C1 are 27-29 mg/kg. At the higher ascorbate intake CT was 92-114 mg/kg, but decreased by 34% at the lower feeding rate to 51-62 mg/kg. Data indicate that there are two or more body pools that include a store of C2, which is readily interconverted in metabolizing tissues to and from C1. Since air and water stable C2 is antiscorbutic for fish, it is the preferred form of ascorbate for fish feeds.

• Research Organization: Univ. of Washington, Seattle
• OSTI ID: 6131998
• Journal Information: J. Nutr.; (United States), Journal Name: J. Nutr.; (United States) Vol. 114:6; ISSN JONUA
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
AQUATIC ORGANISMS
ASCORBIC ACID
BIOLOGICAL HALF-LIFE
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BODY
BODY FLUIDS
BRAIN
CARBON 14 COMPOUNDS
CENTRAL NERVOUS SYSTEM
CLEARANCE
DIET
DIGESTIVE SYSTEM
DISTRIBUTION
EXCRETION
FECES
FISHES
GLANDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIVER
MATERIALS
METABOLISM
NERVOUS SYSTEM
ORGANS
TISSUE DISTRIBUTION
TRACER TECHNIQUES
TROUT
URINE
VERTEBRATES
VITAMINS
WASTES