Demonstration of specific E-type prostaglandin receptors using enriched preparations of canine parietal cells and (/sup 3/H)misoprostol free acid

Journal Article · Mon Jul 27 04:00:00 EDT 1987 · Am. J. Med.; (United States)

OSTI ID:6125815

Tsai, B S; Kessler, L K; Schoenhard, G; Collins, P W; Bauer, R F

High-affinity, E-type prostaglandin binding sites in enriched canine parietal cell preparations were identified with (/sup 3/H) misoprostol free acid, a prostaglandin E1 analogue. Saturable, reversible, and highly stereospecific binding was identified, with approximately 8000 binding sites per cell. Prostaglandin I and F bound weakly, and cimetidine and histamine did not bind. The results indicate that (/sup 3/H) misoprostol free acid binds to E-type prostaglandin receptors, which suggests that the ulcer-healing inhibition of gastric acid secretion by misoprostol results from its interaction with a specific E-type prostaglandin receptor.

• Research Organization: G.D. Searle and Company, Skokie, IL
• OSTI ID: 6125815
• Journal Information: Am. J. Med.; (United States), Journal Name: Am. J. Med.; (United States) Vol. 83:1A; ISSN AJMEA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINES
ANIMALS
AZOLES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MATERIALS
BODY FLUIDS
DOGS
GASTRIC ACID
HETEROCYCLIC COMPOUNDS
HISTAMINE
IMIDAZOLES
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
MAMMALS
MATERIALS
MEMBRANE PROTEINS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PATHOLOGICAL CHANGES
PROSTAGLANDINS
PROTEINS
REACTION KINETICS
RECEPTORS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ULCERS
VERTEBRATES