Application of arabinofuranosyl cytosine in the kinetic analysis and quantitation of DNA repair in human cells after ultraviolet irradiation
Journal Article
·
· Biophys. J.; (United States)
We have developed a technique whereby 3-h pulses of arabinofuranosyl cytosine (ara-C) and hydroxyurea (HU) are used to analyze the kinetics of repair with time after ultraviolet irradiation in human fibroblasts. We demonstrate that this technique offers a significant improvement over existing repair assays in its ability to visualize between 57 and 100% of all sites undergoing repair in a given period of time. In addition, kinetic analyses of repair are more easily made and yield more information than techniques such as repair replication or unscheduled DNA synthesis. We have also examined the nature of the inhibition event by ara-C and have determined that repair breaks accumulate in the presence of ara-C and HU only up to a certain time beyond which no further breaks appear. The time needed to reach this saturation point depends on the number of sites undergoing repair during the treatment time. This observation is discussed with respect to a possible mechanism of excision repair inhibition by ara-C and HU.
- Research Organization:
- Oak Ridge National Lab., TN
- DOE Contract Number:
- W-7405-ENG-26
- OSTI ID:
- 6123493
- Journal Information:
- Biophys. J.; (United States), Journal Name: Biophys. J.; (United States) Vol. 35:2; ISSN BIOJA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Inhibition of DNA excision repair in human cells by Arabinofuranosyl cytosine: effect on normal and xeroderma pigmentosum cells
Nick translation - a new assay for monitoring DNA damage and repair in cultured human fibroblasts
Ultraviolet and chemical induced DNA repair in human cells assayed by bromodeoxyuridine photolysis or cytosine arabinoside arrest
Journal Article
·
Sun Dec 31 23:00:00 EST 1978
· Mol. Pharmacol.; (United States)
·
OSTI ID:5415215
Nick translation - a new assay for monitoring DNA damage and repair in cultured human fibroblasts
Journal Article
·
Mon Dec 31 23:00:00 EST 1984
· Environ. Mutagen.; (United States)
·
OSTI ID:6525866
Ultraviolet and chemical induced DNA repair in human cells assayed by bromodeoxyuridine photolysis or cytosine arabinoside arrest
Conference
·
Sun Dec 31 23:00:00 EST 1978
·
OSTI ID:5731455
Related Subjects
550200* -- Biochemistry
560111 -- Radiation Effects on Biochemicals-- In Vitro-- (-1987)
560121 -- Radiation Effects on Cells-- External Source-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMIDES
AMINES
ANIMAL CELLS
AZINES
BIOASSAY
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CELL CULTURES
CONNECTIVE TISSUE CELLS
CYTOSINE
DNA
ELECTROMAGNETIC RADIATION
ENZYME INHIBITORS
FIBROBLASTS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
HYDROXYUREA
KINETICS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PYRIMIDINES
RADIATION EFFECTS
RADIATIONS
RADIOSENSITIVITY EFFECTS
REACTION KINETICS
RECOVERY
REPAIR
SOMATIC CELLS
ULTRAVIOLET RADIATION
560111 -- Radiation Effects on Biochemicals-- In Vitro-- (-1987)
560121 -- Radiation Effects on Cells-- External Source-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMIDES
AMINES
ANIMAL CELLS
AZINES
BIOASSAY
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CELL CULTURES
CONNECTIVE TISSUE CELLS
CYTOSINE
DNA
ELECTROMAGNETIC RADIATION
ENZYME INHIBITORS
FIBROBLASTS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
HYDROXYUREA
KINETICS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PYRIMIDINES
RADIATION EFFECTS
RADIATIONS
RADIOSENSITIVITY EFFECTS
REACTION KINETICS
RECOVERY
REPAIR
SOMATIC CELLS
ULTRAVIOLET RADIATION