Ultraviolet and chemical induced DNA repair in human cells assayed by bromodeoxyuridine photolysis or cytosine arabinoside arrest
Conference
·
OSTI ID:5731455
The bromodeoxyuridine photolysis assay of DNA damage in human cells permits an estimate of both the number of repaired regions in the DNA and the size of the average repaired region - the patch size. The antineoplastic agent arabinofuranosyl cytosine (ara-C) can also be employed to assay the magnitude of repair since this agent appears to block rejoining of single-strand incisions made in the DNA during the initial step of repair. Thus, the number of incisions can be accumulated. The ara-C effect is dependent on the presence of hydroxyurea. Both assays can be employed for the study of physical or chemical DNA damages. Results comparing these assays are presented.
- Research Organization:
- Oak Ridge National Lab., TN (USA)
- DOE Contract Number:
- W-7405-ENG-26
- OSTI ID:
- 5731455
- Report Number(s):
- CONF-790992-1
- Country of Publication:
- United States
- Language:
- English
Similar Records
Nick translation - a new assay for monitoring DNA damage and repair in cultured human fibroblasts
Inhibition of DNA excision repair in human cells by Arabinofuranosyl cytosine: effect on normal and xeroderma pigmentosum cells
Sensitivity of excision repair in normal human, xeroderma pigmentosum variant and Cockayne's syndrome fibroblasts to inhibition by cytosine arabinoside
Journal Article
·
Mon Dec 31 23:00:00 EST 1984
· Environ. Mutagen.; (United States)
·
OSTI ID:6525866
Inhibition of DNA excision repair in human cells by Arabinofuranosyl cytosine: effect on normal and xeroderma pigmentosum cells
Journal Article
·
Sun Dec 31 23:00:00 EST 1978
· Mol. Pharmacol.; (United States)
·
OSTI ID:5415215
Sensitivity of excision repair in normal human, xeroderma pigmentosum variant and Cockayne's syndrome fibroblasts to inhibition by cytosine arabinoside
Journal Article
·
Sat Aug 01 00:00:00 EDT 1981
· J. Cell. Physiol.; (United States)
·
OSTI ID:6162446
Related Subjects
550201 -- Biochemistry-- Tracer Techniques
550301 -- Cytology-- Tracer Techniques
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
560301 -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANTIMETABOLITES
ANTIMITOTIC DRUGS
AZINES
BIOASSAY
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BROMOURACILS
BUDR
CELL CULTURES
CHEMICAL REACTIONS
COMPARATIVE EVALUATIONS
CONNECTIVE TISSUE CELLS
DATA
DATA FORMS
DECOMPOSITION
DNA
DRUGS
ELECTROMAGNETIC RADIATION
EXPERIMENTAL DATA
FIBROBLASTS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
INFORMATION
IRRADIATION
ISOLATED VALUES
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
NUMERICAL DATA
ORGANIC BROMINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHOTOCHEMICAL REACTIONS
PHOTOLYSIS
PYRIMIDINES
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
RIBOSIDES
SOMATIC CELLS
STRAND BREAKS
ULTRAVIOLET RADIATION
URACILS
XP CELLS
550301 -- Cytology-- Tracer Techniques
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
560301 -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANTIMETABOLITES
ANTIMITOTIC DRUGS
AZINES
BIOASSAY
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BROMOURACILS
BUDR
CELL CULTURES
CHEMICAL REACTIONS
COMPARATIVE EVALUATIONS
CONNECTIVE TISSUE CELLS
DATA
DATA FORMS
DECOMPOSITION
DNA
DRUGS
ELECTROMAGNETIC RADIATION
EXPERIMENTAL DATA
FIBROBLASTS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
INFORMATION
IRRADIATION
ISOLATED VALUES
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
NUMERICAL DATA
ORGANIC BROMINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHOTOCHEMICAL REACTIONS
PHOTOLYSIS
PYRIMIDINES
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
RIBOSIDES
SOMATIC CELLS
STRAND BREAKS
ULTRAVIOLET RADIATION
URACILS
XP CELLS