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Chloroform markedly stimulates the phosphorylation of myelin basic proteins

Journal Article · · Biochem. Biophys. Res. Commun.; (United States)
We have investigated the effect of chloroform on the phosphorylation of myelin basic proteins because tumor-promoting agents such as phorbol esters and chloroform are known to enhance the activity of protein kinase C. We report that the presence of chloroform, at a concentration known to enhance protein kinase C activity, stimulated the phosphorylation of myelin basic proteins 15-17 fold over control conditions. The phosphorylation of a 50 kiloDalton myelin protein was also stimulated but to a lesser extent. The concentration of chloroform required for the maximal phosphorylation of myelin basic proteins and the 50 kiloDalton protein was approximately 2% (v/v).
Research Organization:
McGill Univ., Montreal, Quebec
OSTI ID:
6121721
Journal Information:
Biochem. Biophys. Res. Commun.; (United States), Journal Name: Biochem. Biophys. Res. Commun.; (United States) Vol. 146:3; ISSN BBRCA
Country of Publication:
United States
Language:
English