Lack of induction of single-strand breaks in mammalian cells by sodium azide and its proximal mutagen
The mutagenicity of sodium azide in both higher plants and bacteria is well documented. However, in mammalian cells, research on the effects of azide on gene mutations has produced conflicting results. Furthermore, no research has been conducted on the effects of azide and its proximal mutagen (mutagenic metabolite) on single-strand breaks. The experiments herein were designed to overcome this lack of information on azide mutagenicity and to evaluate the potential hazard of azide exposure to man. Chinese hamster V/sub 79/ cells were treated with either azide or its proximal mutagen(s) for 2 h or 6 h respectively and analyzed by alkaline elution for single-strand breaks. The data showed that there was no effect of either azide or the proximal mutagen(s) on single-strand DNA breaks nor was there any indication that azide or the proximal mutagen(s) induced DNA-protein crosslinks. The data suggest that neither azide nor its proximal mutagen(s) interact directly with DNA and suggest that the methods may be applied to any specified population and dose scenario.
- Research Organization:
- Washington State Univ., Pullman (USA). Coll. of Agriculture
- DOE Contract Number:
- AT06-76EV72002
- OSTI ID:
- 6111002
- Report Number(s):
- DOE/EV/72002-56; ON: DE82001721
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ANIMAL CELLS
GENE MUTATIONS
AZIDES
MUTAGENESIS
DNA
CROSS-LINKING
STRAND BREAKS
CELL CULTURES
HAMSTERS
METABOLITES
PROTEINS
SODIUM COMPOUNDS
ALKALI METAL COMPOUNDS
ANIMALS
CHEMICAL REACTIONS
MAMMALS
MUTATIONS
NITROGEN COMPOUNDS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
POLYMERIZATION
RODENTS
VERTEBRATES
560301* - Chemicals Metabolism & Toxicology- Cells- (-1987)